Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from stimulated or unstimulated human CD4+ T cells incubated with edelfosine


ABSTRACT: The cytotoxic drug edelfosine is a synthetic analog of 2-lysophosphatidylcholine. Edelfosine is incorporated by highly proliferating cells, e.g. activated immune cells. It is unknown if the described mechanisms for edelfosine action attained by in vitro approaches exclusively contribute to the observed EAE-amelioration or if edelfosine may exert additional, probably more general and possibly immunoablative effects within the setting of autoimmunity. We used microarray analysis in order to confirm proposed mechanisms of edelfosine action, but also to discover novel effects of edelfosine in the context of immune cells. For gene-expression analysis enriched CD4+ T cells obtained from Buffy Coats were plated at 200,000 cells/well in 96-well plates. Cells were cultured for 30 h in X-Vivo 15 medium or X-Vivo 15 medium supplemented with 3.3 ug/ml edelfosine, 10 ug/ml edelfosine, bead particles coated with antibodies against CD2, CD3, and CD28 (Miltenyi), or 3.3 ug/ml edelfosine in combination with bead particles coated with antibodies against CD2, CD3 and CD28, respectively.

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Streichert 

PROVIDER: E-GEOD-44392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The orally available, synthetic ether lipid edelfosine inhibits T cell proliferation and induces a type I interferon response.

Abramowski Pierre P   Otto Benjamin B   Martin Roland R  

PloS one 20140325 3


The drug edelfosine is a synthetic analog of 2-lysophosphatidylcholine. Edelfosine is incorporated by highly proliferating cells, e.g. activated immune cells. It acts on cellular membranes by selectively aggregating the cell death receptor Fas in membrane rafts and interference with phosphatidylcholine (PC) synthesis with subsequent induction of apoptosis. Edelfosine has been proposed for the treatment of autoimmune diseases like multiple sclerosis (MS). Earlier studies on the animal model of MS  ...[more]

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