POF (MO 459).D.mel 3rd Instar Larvae Nuclei POF Mutant
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
SUBMITTER: DCC modENCODE
PROVIDER: E-GEOD-44496 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:modENCODE_submission_3686 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: POF mutant Developmental Stage: 3rd Instar Larvae; Genotype: POF [D119]/POF [D119]; Transgene: deletion; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain POF mutant Antibody H3K36me3 abcam (target is H3K36me3); Developmental Stage 3rd Instar Larvae
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