Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

33 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiple tissue expression data from inbreds and F1 of CAST, PWK, and WSB

ABSTRACT: We create catalogues of genes showing significant strain, parent-of-origin, dominance, sex effect in inbreds and reciprocal F1 hybrids of three wild-derived strains (CAST, PWK, WSB) across 4 different tissues (brain, kidney, liver, and lung) We used microarrays to validate the Brain results of RNAseq from inbred and F1 of 3 by 3 diallel. Brain, liver, kidney and lung RNA from the same mice used for RNAseq were hybridized to Affymetrix Mouse Gene 1.1 ST 96-Array Plate arrays using a GeneTitan instrument from Affymetrix.

ORGANISM(S): Mus musculus

SUBMITTER: Yunjung Kim

PROVIDER: E-GEOD-44555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Multiple tissue expression data from inbreds and F1 of CAST, PWK, and WSB

Project description:We create catalogues of genes showing significant strain, parent-of-origin, dominance, sex effect in inbreds and reciprocal F1 hybrids of three wild-derived strains (CAST, PWK, WSB) across 4 different tissues (brain, kidney, liver, and lung) We used microarrays to validate the Brain results of RNAseq from inbred and F1 of 3 by 3 diallel.

2013-05-01 | GSE44555 | GEO

Increased Rho GTPase activity is associated with decreased tumor inhibitory activity of immortalized fibroblasts

Project description:Clone (Whirly) of human BJhTERT (human foreskin cell line) cells exposed to PC3 mRFP cells Clones (Whirly) of human BJhTERT (human foreskin cell line) cells exposed to PC3 mRFP cells were analyzed with Affymetrix Whole Target (WT) Assay (GeneTitan plate format Gene ST 1.1)

2014-04-17 | E-GEOD-56832 | biostudies-arrayexpress

Confrontation of fibroblasts with cancer cells in vitro: gene network analysis of transcriptome changes and differential capacity to inhibit tumor growth

Project description:Different fibroblast cells (eight in total) with different inhibitory capacity were analyzed and compared for their gene expression profile by whole genome microarray. Different fibroblast cells (eight in total) were analyzed with Affymetrix Whole Target (WT) Assay (GeneTitan plate format Gene ST 1.1) in order to find out gene expression differences.

2014-05-01 | E-GEOD-57199 | biostudies-arrayexpress

Increased Rho GTPase activity is associated with decreased tumor inhibitory activity of immortalized fibroblasts

Project description:Two clones (Whirly and Crossy) of human BJhTERT (human foreskin cell line) cells with different inhibitory capacity were analyzed and compared for their gene expression profile by whole genome microarray. Two clones (Whirly and Crossy) of human BJhTERT (human foreskin cell line) cells were analyzed with Affymetrix Whole Target (WT) Assay (GeneTitan plate format Gene ST 1.1) in order to find out gene expression differences.

2014-03-08 | E-GEOD-55681 | biostudies-arrayexpress

Transcription profiling of human multiple myeloma cell lines treated with EZH2 inhibitor E7438 and DMSO controls

Project description:We used a total of 10 human multiple myeloma cell lines for the study (KMS-20, KMS-28BM, MOLP-8, RPMI-8226, U-266, KMS-11, KMS-12-PE, KMS-34, LP-1 and NCI-H929). Cells (2 x 105 per well) were seeded into six-well culture plates in the appropriate culture media 24 h before treatment. Five replicate wells were then exposed to 2 uM of E7438 or control DMSO for 3 days. Cells were collected and RNA was extracted using RNeasy kit (Qiagen) according to the manufacturer’s instructions. For each sample, 250ng of total RNA was amplified using the Affymetrix GeneChip WT PLUS Reagent Kit according to the protocol described in User Manual Target Preparation for GeneChip Whole Transcript (WT) Expression Arrays (P/N 703174 Rev. 2). An Affymetrix Human Gene 2.1 ST 96-array plate was hybridized with 3µg of fragmented and labeled ss cDNA, washed, stained, and scanned according to the protocol described in User Manual GeneTitan®Instrument User Guide for Expression Arrays Plates (P/N 702933 Rev.1) and Affymetrix® GeneChip® Command Console™ User’s Guide (P/N 702569 Rev.9) using the Affymetrix GeneTitan instrument.

2016-01-11 | E-MTAB-3540 | biostudies-arrayexpress

RNAseq of 16 North American Beaver (Castor canadensis) tissues

Project description:RNAseq from the following tissue samples: skeletal muscle; kidney; spleen; ovaries; placenta; castor gland; tail; toe webbing; whole blood; brain; lung; liver; heart; stomach; tongue; intestine

2019-07-02 | E-MTAB-8038 | biostudies-arrayexpress

Expression data from IMQ-induced psoriasis-like skin inflammation in miR-146a-/- and C57BL6J mice

Project description:miR-146a acts as a negative feedback regulator of inflammation. To investigate the role of miR-146a in psoriasis psoriasiform skin inflammation was indeuced in Mir-146a-/- and wild type mice (C57BL6J) by topical applciation of imiquimod (IMQ)-cream (Aldara). Gene expression profiling (Affymetrix) was used to identify transcriptomic changes associated with psoriasis-like skin inflammation in wild type vs. miR-146a -/-mice. A daily topical dose of 31.25 mg of Aldara cream (5% IMQ) was applied on the right ear of miR-146a -/- and C57BL/6 mice on three consecutive days to induce psorisis-like skin inflammation. Mice were sacrificed at day 4. Ear flaps were collected for total RNA extraction and hybridization on Affymatrix GeneTitan plate format Gene ST 2.1 (mouse).

2016-02-19 | E-GEOD-78057 | biostudies-arrayexpress

North American Beaver (Castor canadensis) RNAseq 16 pooled tissue samples.

Project description:RNAseq from Illumina MiSeq 75bp paired end for transcriptome assembly. The following tissue samples were pooled: skeletal muscle; kidney; spleen; ovaries; placenta; castor gland; tail; toe webbing; whole blood; brain; lung; liver; heart; stomach; tongue; intestine

2017-12-24 | E-MTAB-6258 | biostudies-arrayexpress