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The Translation Initiation Factor eIF3h Targets Specific Transcripts to Polysomes during Embryogenesis

ABSTRACT: We have sequenced the polysome-associated translating mRNAs from stage-matched wild-type and eif3ha morphant embryos at ~24 hpf stage to identify transcripts translationally regulated by eIF3ha. As a control, we have also sequenced total mRNAs from the stage-matched wild-type and eif3ha morphants as well at ~ 24 hpf. Polysome-associated mRNAs were isolated from 300 zebrafish embryos. Total RNA was isolated from 50 zebrafish embryos. Single 36-base pair reads were sequenced on the Illumina Genome Analyzer Iix.

ORGANISM(S): Danio rerio

SUBMITTER: Todd Evans 

PROVIDER: E-GEOD-44584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis.

Choudhuri Avik A   Maitra Umadas U   Evans Todd T  

Proceedings of the National Academy of Sciences of the United States of America 20130528 24

Eukaryotic translation initiation factor 3 (eIF3) plays a central role in translation initiation and consists of five core (conserved) subunits present in both budding yeast and higher eukaryotes. Higher eukaryotic eIF3 contains additional (noncore or nonconserved) subunits of poorly defined function, including sub-unit h (eIF3h), which in zebrafish is encoded by two distinct genes (eif3ha and eif3hb). Previously we showed that eif3ha encodes the predominant isoform during zebrafish embryogenesi  ...[more]

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