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Independent Regulation of Metabolism but Coordinated Control of Tissue Development by Epidermal Specific Transcription Factors in Caenorhabditis elegans


ABSTRACT: We identified target genes for NHR-25 by ChIP-seq at L1 stage of C. elegans. Transcription factor genes were tagged with GFP and their expression examined at L1 stage. Since there are no direct target genes known for NHR-25 that can be used for assessment of enrichment efficiency by quantitative PCR (qPCR), we chose to repeat ChIP-seq experiment of another GFP tagged transcription factor, PHA-4 for which the ChIP-seq was performed during a pilot experiment of modENCODE project using the same transgenic strain and antibody (a gift from Tony Hyman lab). pha-4 and nhr-25 transgenic worm were studied in Fed L1 stage.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Jiaofang Shao 

PROVIDER: E-GEOD-44710 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Collaborative regulation of development but independent control of metabolism by two epidermis-specific transcription factors in Caenorhabditis elegans.

Shao Jiaofang J   He Kan K   Wang Hao H   Ho Wing Sze WS   Ren Xiaoliang X   An Xiaomeng X   Wong Ming Kin MK   Yan Bin B   Xie Dongying D   Stamatoyannopoulos John J   Zhao Zhongying Z  

The Journal of biological chemistry 20131006 46


Cell fate specification is typically initiated by a master regulator, which is relayed by tissue-specific regulatory proteins (usually transcription factors) for further enforcement of cell identities, but how the factors are coordinated among each other to "finish up" the specification remains poorly understood. Caenorhabditis elegans epidermis specification is initiated by a master regulator, ELT-1, that activates its targets, NHR-25 and ELT-3, two epidermis-specific transcription factors that  ...[more]

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