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Expression analyses of red5-2 mutant cells

ABSTRACT: Investigation of whole genome gene expression level changes in red5-2, compared to the wild-type strain. The mutants analyzed in this study will be described in Sugiyama and Sugiyama. A mRNA profiling study using total RNA recovered from wild-type culture of fission yeast and red5-2 mutant cultures of fission yeast. Each chip measures the expression level of 4.997 genes from S.pombe with fourteen 60-mer probe pairs (PM/MM) per gene, with seven-fold technical redundancy.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Tomoyasu Sugiyama

PROVIDER: E-GEOD-44714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.

Sugiyama Tomoyasu T Wanatabe Nobuyoshi N Kitahata Eri E Tani Tokio T Sugioka-Sugiyama Rie R

Nucleic acids research 20130508 13

Zinc-finger domains are found in many nucleic acid-binding proteins in both prokaryotes and eukaryotes. Proteins carrying zinc-finger domains have important roles in various nuclear transactions, including transcription, mRNA processing and mRNA export; however, for many individual zinc-finger proteins in eukaryotes, the exact function of the protein is not fully understood. Here, we report that Red5 is involved in efficient suppression of specific mRNAs during vegetative growth of Schizosacchar ...[more]

PMID: 23658229

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