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Defining a Th9 transcriptome

ABSTRACT: Microarray analyses were performed to compare gene expression in cultured mouse Th9, Th2 and Treg cells and resting versus activated Th9 cells. Three replicates were analyzed for each culture condition; Th9 unstim, Th2 unstim, Treg unstim, Th9 stim

ORGANISM(S): Mus musculus

SUBMITTER: Mark Kaplan

PROVIDER: E-GEOD-44937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Th9 cell development requires a BATF-regulated transcriptional network.

Jabeen Rukhsana R Goswami Ritobrata R Awe Olufolakemi O Kulkarni Aishwarya A Nguyen Evelyn T ET Attenasio Andrea A Walsh Daniel D Olson Matthew R MR Kim Myung H MH Tepper Robert S RS Sun Jie J Kim Chang H CH Taparowsky Elizabeth J EJ Zhou Baohua B Kaplan Mark H MH

The Journal of clinical investigation 20131101 11

T helper 9 (Th9) cells are specialized for the production of IL-9, promote allergic inflammation in mice, and are associated with allergic disease in humans. It has not been determined whether Th9 cells express a characteristic transcriptional signature. In this study, we performed microarray analysis to identify genes enriched in Th9 cells compared with other Th subsets. This analysis defined a transcriptional regulatory network required for the expression of a subset of Th9-enriched genes. The ...[more]

PMID: 24216482

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2022-10-31 | E-MTAB-12199 | biostudies-arrayexpress

Phenotypes of Th lineages generated by the commonly used activation with anti-CD3/CD28 antibodies differ from those generated by the physiological activation with the specific antigen

Project description:T-helper (Th) lineages have been generated in vitro by activating CD4 cells with anti-CD3/CD28 antibodies during polarization. Physiologically, however, the generation of Th lineages is by activation with the specific antigen presented by antigen-presenting cells (APC). Here, we used TCR-transgenic mice to compare the phenotypes of Th1, Th9 and Th17 lineages when generated by either one of the two activation modes. Lineage Th cells specific against hen egg lysozyme (HEL), were adoptively transferred into recipient mice transgenically expressing HEL in their lens. Remarkable differences were found between lineages of Th1, Th9, or Th17, generated by either one of the two modes in their capacities to migrate to and proliferate in the recipient spleen and, importantly, to induce inflammation in the recipient mouse eyes. Substantial differences were also observed between the lineage pairs in their transcript expression profiles of certain chemokines and chemokine receptors. Surprisingly, however, close similarities were observed between the transcript expression profiles of lineages of the three phenotypes, activated by the same mode. Furthermore, Th cell lineages generated by the two activation modes differed considerably in their pattern of gene expression, as monitored by microarray analysis, but exhibited commonality with lineages of other phenotypes generated by the same activation mode. This study thus shows that (i) Th lineages generated by activation with anti-CD3/CD28 antibodies differ from lineages generated by antigen/APC and (ii) the mode of activation determines to a large extent the expression profile of major transcripts NaM-CM-/ve CD4+ T cells purified from spleen and lymph node cells of 3A9 mice were activated and polarized toward Th1, Th9, and Th17 lineages, under either plate bound anti-CD3/anti-CD28 (PBAB) or APC presented HEL protein (HA).

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2022-10-31 | E-MTAB-12197 | biostudies-arrayexpress

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2016-06-14 | E-GEOD-79607 | biostudies-arrayexpress

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