Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Real-time quantitative PCR analysis of rat diaphragm before and after acute cervical spinal cord injury

ABSTRACT: Male Sprague Dawley adult rats were subjected to a cervical hemisection at C2 (C2HS). Costal diaphragm (both ipsilesional and contralesional sides) were assessed under control conditions (sham surgery) and at 1 and 7 days post-C2HS. We used SA Biosciences Rat Skeletal Muscle Development and Disease RT2 Profiler PCR Array to quantitate gene expression of muscle atrophy and regeneration-relevant genes from the uninjured and injured tissues on the indicated sides and at the indicated timepoints post-lesion. qPCR gene expression profiling. Tissue was derived from the costal diaphragm from control animals, and from contralesional and ipsilesional sides at days 1 and 7 post-lesion. RNA was extracted from flash-frozen tissue (TRIzol reagent) and quantitated via spectrophotometry. Equal amount total RNA from each donor was pooled prior to gene expression analysis. N=3 or 4 rats per condition. Four genes in the array study appeared to undergo unphysiologic increases in expression following SCI: Mmp9, Leptin, activin A and alpha actinin. For these genes, expression levels in control tissue were detectable but extremely low, thus fold-increases following SCI are numerically exaggerated. Therefore, we cannot explicitly comment on the physiologic scale of this response

ORGANISM(S): Rattus norvegicus

SUBMITTER: Heather Ross

PROVIDER: E-GEOD-45021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rapid diaphragm atrophy following cervical spinal cord hemisection.

Gill L C LC Ross H H HH Lee K Z KZ Gonzalez-Rothi E J EJ Dougherty B J BJ Judge A R AR Fuller D D DD

Respiratory physiology & neurobiology 20131214

A cervical (C2) hemilesion (C2Hx), which disrupts ipsilateral bulbospinal inputs to the phrenic nucleus, was used to study diaphragm plasticity after acute spinal cord injury. We hypothesized that C2Hx would result in rapid atrophy of the ipsilateral hemidiaphragm and increases in mRNA expression of proteolytic biomarkers. Diaphragm tissue was harvested from male Sprague-Dawley rats at 1 or 7 days following C2Hx. Histological analysis demonstrated reduction in cross-sectional area (CSA) of type ...[more]

PMID: 24341999

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Dataset Information

Real-time quantitative PCR analysis of rat diaphragm before and after acute cervical spinal cord injury

Publications

Rapid diaphragm atrophy following cervical spinal cord hemisection.

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