Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Diel growth of Thalassiosira pseudonana


ABSTRACT: Transcript levels of all T. pseudonana genes was measured every twelve hours throughout the batch (non-chemostatic) growth of axenic cells grown in large glass bioreactors on a 12hr:12hr dark:light cycle for five days. The data were analyzed to reveal the physiological and regulatory changes that recurred in this diatom when transitioning between dark and light conditions, as well as from exponential phase to stationary, nutrient limited conditions. The longitudinal experiment was performed with two replicates, at 400 and 800ppm CO2. Two growth experiments were conducted, with 10 and 9 longitudinal samples collected from each experiment, respectively. Two-color arrays were used with dye flips for labeling. A common internal reference sample was used for one channel on each array. Expression changes for longitudinal analysis were calculated as the difference from the mean log2 expression ratio for each to the common reference sample, for each gene, over all samples within an experiment. For the analysis of diel states in T. pseudonana, samples from the two series were matched according to the time from innoculation, and divided into four classes: dawn samples (taken at the end of each dark phase), dusk samples (taken at the end of each light phase), exponential samples (the first five samples in each series prior to a drop in growth rate on Day 3), and stationary samples (all samples including following the drop in growth rate on Day 3).

ORGANISM(S): Thalassiosira pseudonana CCMP1335

SUBMITTER: Justin Ashworth 

PROVIDER: E-GEOD-45252 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2023-05-10 | PXD027392 | Pride
2013-04-18 | GSE45252 | GEO
2014-03-25 | E-GEOD-56132 | biostudies-arrayexpress
2023-02-06 | GSE213604 | GEO
2015-11-01 | E-GEOD-36677 | biostudies-arrayexpress
2011-07-01 | E-GEOD-27521 | biostudies-arrayexpress
2010-05-26 | E-GEOD-9697 | biostudies-arrayexpress
2008-02-01 | E-MEXP-1269 | biostudies-arrayexpress
2013-08-23 | E-GEOD-48620 | biostudies-arrayexpress
2022-11-21 | E-MEXP-593 | biostudies-arrayexpress