RNAseq analysis of the serotype M1 group A Streptococcus strain MGAS2221
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ABSTRACT: We recovered RNA from exponential and stationary phase cell cultures of MGAS2221 and utilized two distinct methods to remove rRNA from our samples, the RiboZero kit from Epicenter, and the terminator- 5--Phosphate-Dependent Exonuclease (TEX). This gave us 4 distinct RNAs with which we performed RNAseq analysis. Both rRNA removal methods worked well, with only a small percentage of the final total read counts being derived from rRNA sequences. A single group A Streptococcus strain was investigated.
ORGANISM(S): Streptococcus pyogenes
SUBMITTER: Paul Sumby
PROVIDER: E-GEOD-45325 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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