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Next generation sequencing analysis of microRNAs in brown preadipocytes and in vitro differentiated mature brown fat cells

ABSTRACT: We performed a genome-wide deep sequencing analysis of the microRNAs abundant in mesenchymal stem cells (MSCs) derived from murine brown adipose tissue and in in vitro differentiated mature brown adipocytes. Several microRNAs were identified as differentially regulated when comparing datasets from MSCs vs. mature fat cells. These microRNAs may have an implication in the regulation of adipogenesis as well as thermogenesis in brown adipose tissue (BAT). Examination of BAT-derived MSCs (BAT-MSC; 1 sample) and in vitro differentiated mature brown fat cells (BAT-DIFF; 1 sample) vertis biotechnologie AG, D-85354 Freising, Germany (library construction and sequencing)

ORGANISM(S): Mus musculus

SUBMITTER: Franziska Siegel

PROVIDER: E-GEOD-45499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-155 regulates differentiation of brown and beige adipocytes via a bistable circuit.

Chen Yong Y Siegel Franziska F Kipschull Stefanie S Haas Bodo B Fröhlich Holger H Meister Gunter G Pfeifer Alexander A

Nature communications 20130101

Brown adipocytes are a primary site of energy expenditure and reside not only in classical brown adipose tissue but can also be found in white adipose tissue. Here we show that microRNA 155 is enriched in brown adipose tissue and is highly expressed in proliferating brown preadipocytes but declines after induction of differentiation. Interestingly, microRNA 155 and its target, the adipogenic transcription factor CCAAT/enhancer-binding protein β, form a bistable feedback loop integrating hormonal ...[more]

PMID: 23612310

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