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Expression analysis of Yarrowia lipolytica Y4184 snf1 mutant

ABSTRACT: Investigation of whole genome gene expression level changes in a Yarrowia lipolytica Y4184 snf1 mutant, compared to the Y4184U+. The Y4184 is an engineered strain to produce eicosapentaenoic acid (EPA) via expression of a M-NM-^T9 elongase/M-NM-^T8 desaturase pathway, and is derived from Yarrowia lipolytica ATCC#20362. A six chip study using total RNA recovered from three separate cultures of Yarrowia lipolytica Y4184Ura+ strains and three separate cultures of Y4184 snf1 mutant strains in which YlSNF1 gene is deleted entirely. Each chip measures the expression level of 6708 genes from Yarrowia lipolytica ATCC#20362 with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

ORGANISM(S): Yarrowia lipolytica

SUBMITTER: Seung-Pyo Hong

PROVIDER: E-GEOD-45611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Snf1 is a regulator of lipid accumulation in Yarrowia lipolytica.

Seip John J Jackson Raymond R He Hongxian H Zhu Quinn Q Hong Seung-Pyo SP

Applied and environmental microbiology 20130920 23

In the oleaginous yeast Yarrowia lipolytica, de novo lipid synthesis and accumulation are induced under conditions of nitrogen limitation (or a high carbon-to-nitrogen ratio). The regulatory pathway responsible for this induction has not been identified. Here we report that the SNF1 pathway plays a key role in the transition from the growth phase to the oleaginous phase in Y. lipolytica. Strains with a Y. lipolytica snf1 (Ylsnf1) deletion accumulated fatty acids constitutively at levels up to 2. ...[more]

PMID: 24056466

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