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MiR-221 mediated gene expression in human PCa cells

ABSTRACT: MiR-221 overexpression leads to activation of apoptosis, growth arrest and reduced invasivness in PCa cells. Interaction of miR-221 with potential target genes was analyzed by a genome wide expression profiling.. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real Time RT-PCR assay (IRF1, IRF2 SOCS3, STAT1), and Western Blotting. In total, 282 genes were upregulated and 64 downregulated based on a more than 2-fold difference to untransfected PC-3 cells. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, tumorigenesis and inflammation. We confirmed dysregulation of IRF-2 SOCS3, STAT1,IRF9. These results indicate that miR-221 overexpression might lead to activation of the JAK/STAT pathway and downregulation of miR-221 might contribute to tumorigenesis in PCa cells. pre-miR-221 transfected PC-3 cells vs unstimulated control cells - total samples analysed are 4.

ORGANISM(S): Homo sapiens

SUBMITTER: Susanne Kneitz

PROVIDER: E-GEOD-45627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MiR-221 mediated gene expression in human PCa cells

Project description:MiR-221 overexpression leads to activation of apoptosis, growth arrest and reduced invasivness in PCa cells. Interaction of miR-221 with potential target genes was analyzed by a genome wide expression profiling.. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real Time RT-PCR assay (IRF1, IRF2 SOCS3, STAT1), and Western Blotting. In total, 282 genes were upregulated and 64 downregulated based on a more than 2-fold difference to untransfected PC-3 cells. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, tumorigenesis and inflammation. We confirmed dysregulation of IRF-2 SOCS3, STAT1,IRF9. These results indicate that miR-221 overexpression might lead to activation of the JAK/STAT pathway and downregulation of miR-221 might contribute to tumorigenesis in PCa cells.

2014-03-27 | GSE45627 | GEO

Identification of miR-145 targets involved in colon cancer

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2010-10-01 | E-GEOD-18625 | biostudies-arrayexpress

Homo sapiens

Project description:MiR-221 mediated gene expression in human PCa cells

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Project description:Liver cancer progression through increased miR-221 mediated tumorigenesis

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The miR-17~92 family miRNAs regulate non-canonical NFκB pathway by targeting Socs3 to control plasma cell differentiation

Project description:Germinal center (GC) reaction in the lymphoid organs is a pivotal process for humoral immune response. The miR-17~92 family miRNAs have been demonstrated that regulate TFH cell differentiation, GC reaction and antibody response. However, whether they may also have functions in B cell activation and differentiation in GC reaction remain largely unknown. Here we show that mice with deletion of miR-17~92 in B cells exhibited reduced GC B cell formation and profound impaired plasma cell (PC) differentiation as well as decreased antibody response upon protein antigen immunization or chronic LMCV infection. In an in vitro plasma cell differentiation (iGCB) system, we found that miR-17~92 deficiency results in markedly decreased in vitro plasma cell (iPC) differentiation and defective NFB activation. Moreover, we developed and performed a screen by CRISPR/small guide (sg) RNA-mediated gene editing among the target genes of miR-17~92 in iGCB culture system. Intriguingly, Socs3 deletion substantially restored iPC differentiation and NFB activation in miR-17~92 deficient B cells, suggesting that Socs3 acts as a negative regulator of NFκB pathway involved in the regulation of iPC differentiation by miR-17~92. However, IL-21-induced STAT3 activation was not affected by miR-17~92 deficiency during iPC differentiation. Mechanistically, Socs3 interacts with MAP3K14 (NIK) and that promotes the ubiquitination of NIK and subsequent proteasome-mediated degradation of NIK, thereby leading to inhibition of the processing of p100 in non-canonical NFκB pathway and PC differentiation.

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Gene expression profiling in prostate cancer cell lines transfected with miR-33a

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miR-221 is required for endothelial tip cell behaviors during vascular development

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Murine bone marrow-derived mast cells (BMMCs) transduced with pAPM lentiviruses overexpressing miR-221 vs control overexpressing miR-221m.

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Project description:Analysis of gene expression of MCF10A to identify the targets of miR-221 and miR-222 Keywords: MCF10, miR-221, miR-222

2011-06-14 | GSE29327 | GEO