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Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, including follistatin [SuperSAGE]

ABSTRACT: Comparing the transcriptomes of pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni (in biological triplicates) we detected 253 genes to be significantly differeantially (p < 1-10; log2ratios < -0.585 or > +0.585) transcribed. pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni were compared by SuperSAGE (Matsumura et al., 2003; with minor changes: Molina et al., 2008), sequencing Illumina (Solexa)

ORGANISM(S): Schistosoma mansoni

SUBMITTER: Silke Leutner

PROVIDER: E-GEOD-45628 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, such as follistatin [Microarray]

Project description:Transcriptional profiling of S. mansoni pairing-experienced males (EM) vs. pairing unexperienced males (UM) Two conditions (EM vs. UM) with three biological samples of EM and in parallel other three biological samples of UM. For each sample (three EM and three UM) were performed four technical replicas.

2013-09-09 | E-GEOD-44193 | biostudies-arrayexpress

Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, including follistatin [SuperSAGE]

Project description:Comparing the transcriptomes of pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni (in biological triplicates) we detected 253 genes to be significantly differeantially (p < 1-10; log2ratios < -0.585 or > +0.585) transcribed.

2013-09-09 | GSE45628 | GEO

Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, such as follistatin [Microarray]

Project description:Transcriptional profiling of S. mansoni pairing-experienced males (EM) vs. pairing unexperienced males (UM)

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Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, including follistatin

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-09 | E-GEOD-45696 | biostudies-arrayexpress

Gene expression profiling of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)

Project description:Biomphalaria glabrata infection by the Schistosoma mansoni free-swimming miracidium and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and triggers a variety of physiological, biochemical and molecular changes. Here, we describe a genome-wide analysis of the S. mansoni miracidium and developing sporocyst. Keywords: life-cycle, development, host-interaction We generated transcriptomic profiles of the developing larval stages of Schistosoma mansoni using long serial analysis of gene expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. From five libraries, 314,799 SAGE tags were sequenced and resulted in a total of 21,440 unique sequence tags. A total of 254 tags were differentially expressed during “conditioned” development and 236 tags were differentially expressed during “un-conditioned” development. In addition, 53 tags were found to be differentially expressed between 6-day conditioned and unconditioned sporocysts and 42 tags between 20-day conditioned and unconditioned sporocysts.

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