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Changes in gene expression profiles of circulating B cells after influenza vaccination in healthy human subjects

ABSTRACT: Daily sampling of peripheral blood from human subjects vaccinated for influenza was done immediately before vaccination and for 10 days after vaccination. In B cells, 90% of transcriptomic variation in subjects who received influenza vaccine within the previous three years was explained by a single temporal pattern unique to the individual. A common set of 742 genes was strongly correlated with the migration of differentiating plasma cell subtypes. Five subjects, 11 time points per subject (pre-vaccination and daily for 10 days post-vaccination)

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Welle

PROVIDER: E-GEOD-45734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-resolution temporal response patterns to influenza vaccine reveal a distinct human plasma cell gene signature.

Henn Alicia D AD Wu Shuang S Qiu Xing X Ruda Melissa M Stover Michael M Yang Hongmei H Liu Zhiping Z Welle Stephen L SL Holden-Wiltse Jeanne J Wu Hulin H Zand Martin S MS

Scientific reports 20130101

To identify sources of inter-subject variation in vaccine responses, we performed high-frequency sampling of human peripheral blood cells post-vaccination, followed by a novel systems biology analysis. Functional principal component analysis was used to examine time varying B cell vaccine responses. In subjects vaccinated within the previous three years, 90% of transcriptome variation was explained by a single subject-specific mathematical pattern. Within individual vaccine response patterns, a ...[more]

PMID: 23900141

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