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Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from GPR15+CD4+ and GPR15-CD4+ T cells in LILP

ABSTRACT: GPR15 is an orphan G-protein coupled receptor and its expression is abundant among T cells in the large intestine lamina propria. We used microarrays to examine charateristics of GPR15- vs GPR15+ CD4+ T cells in LILP and identified distinct classes of up-regulated genes in GPR15+ CD4+ T cells. We have generated GFP knock-in mice in GPR15 locus and used GFP as a maker for GPR15 expression. In heterozygous mice, GPR15+CD4+ T cells and GPR15-CD4-T cells were sorted and RNA was prepared from them.

ORGANISM(S): Mus musculus

SUBMITTER: Dan Littman

PROVIDER: E-GEOD-45773 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GPR15-mediated homing controls immune homeostasis in the large intestine mucosa.

Kim Sangwon V SV Xiang Wenkai V WV Kwak Changsoo C Yang Yi Y Lin Xiyao W XW Ota Mitsuhiko M Sarpel Umut U Rifkin Daniel B DB Xu Ruliang R Littman Dan R DR

Science (New York, N.Y.) 20130509 6139

Lymphocyte homing, which contributes to inflammation, has been studied extensively in the small intestine, but there is little known about homing to the large intestine, the site most commonly affected in inflammatory bowel disease. GPR15, an orphan heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor, controlled the specific homing of T cells, particularly FOXP3(+) regulatory T cells (Tregs), to the large intestine lamina propria (LILP). GPR15 expression was modulated ...[more]

PMID: 23661644

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