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Transcriptome-profiling (RNA-seq) and Ribosome-profiling (Ribo-seq) of BJ cells treated with Nutlin-3a, an MDM2 inhibitor, which induces p53.

ABSTRACT: We applied in parallel RNA-Seq and Ribosome-profiling analyses to immortalized human primary BJ fibroblast cells in which p53 was induced by Nutlin-3a RNA-seq, using Illumina HiSeq 2000, was applied to BJ cells treated with Nutlin-3a, at 5 timepoints: 0, 2, 4, 6, 19 hrs Ribosome profiling was applied to BJ cells treated with Nutlin-3a, at 5 timepoints: 0, 2, 4, 6, 19 hrs

ORGANISM(S): Homo sapiens

SUBMITTER: Ran Elkon

PROVIDER: E-GEOD-45785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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p53 induces transcriptional and translational programs to suppress cell proliferation and growth.

Loayza-Puch Fabricio F Drost Jarno J Rooijers Koos K Lopes Rui R Elkon Ran R Agami Reuven R

Genome biology 20130417 4

<h4>Background</h4>Cell growth and proliferation are tightly connected to ensure that appropriately sized daughter cells are generated following mitosis. Energy stress blocks cell growth and proliferation, a critical response for survival under extreme conditions. Excessive oncogenic stress leads to p53 activation and the induction of senescence, an irreversible state of cell-cycle arrest and a critical component in the suppression of tumorigenesis. Nutrient-sensing and mitogenic cues converge o ...[more]

PMID: 23594524

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