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Transcription profiling of human B cells at four cconsecutive stages of B cell development

ABSTRACT: Cells from four develppmental stages were purified by FACS from human bone marrow samples Experiment Overall Design: Cells were sorted as follows: PB1: CD34+VpreB+ PB2: VpreB-CD34+ LAR: kappa/lambda-VpreB-large SMA: kappa/lambda-VpreB-small. All cells were CD19+

ORGANISM(S): Homo sapiens

SUBMITTER: Reinhard Hoffmann

PROVIDER: E-GEOD-4590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neutrality, compensation, and negative selection during evolution of B-cell development transcriptomes.

Hoffmann Reinhard R Lottaz Claudio C Kühne Thomas T Rolink Antonius A Melchers Fritz F

Molecular biology and evolution 20070921 12

B cells develop in the mammalian bone marrow through a sequence of precursor stages, which can be ordered by the recombination status of their immunoglobulin loci. This developmental pathway is functionally similar between mice and man. However, whether this similarity is based on usage of the same genes is unknown. We show that large-scale gene expression patterns differ substantially between human and mouse B-cell development. Among 644 genes which were differentially expressed in 4 early stag ...[more]

PMID: 17890238

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