Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 2


ABSTRACT: We developed a method to isolate pure circulating tumor cells (CTC). RNA from such CTCs isolated from the peripheral blood of metastatic breats cnacer patients and gene expression was performed using cDNAmicroarray. we used cDNA array to compare gene expression of CTCs with normal epithelial and breast tumor samples normal peripheral blood, normal epithelium, and CTCs

ORGANISM(S): Homo sapiens

SUBMITTER: Vasu Punj 

PROVIDER: E-GEOD-45963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Circulating tumor cells (CTCs) are prognostic in all stages of breast cancer. However, since they are extremely rare, little is known about the molecular nature of these cells. We report a novel strategy for the isolation and expression profiling of pure populations of CTCs derived from peripheral blood. We developed a method to isolate CTCs based on immunomagnetic capture followed by fluorescence-activated cell sorting (IE/FACS). After assay validation using the BT474 cell line spiked into bloo  ...[more]

Similar Datasets

2014-12-18 | E-GEOD-45964 | biostudies-arrayexpress
2014-12-18 | E-GEOD-45962 | biostudies-arrayexpress
2014-12-18 | E-GEOD-45965 | biostudies-arrayexpress
2013-04-01 | E-GEOD-43379 | biostudies-arrayexpress
2011-12-31 | E-GEOD-26095 | biostudies-arrayexpress
2014-04-03 | E-GEOD-56470 | biostudies-arrayexpress
2010-02-05 | E-GEOD-16380 | biostudies-arrayexpress
2010-11-01 | E-GEOD-19064 | biostudies-arrayexpress
2015-12-16 | E-GEOD-72558 | biostudies-arrayexpress
2015-12-16 | E-GEOD-72557 | biostudies-arrayexpress