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Thermotolerance in B. subtilis

ABSTRACT: During thermotolerance development, B. subtilis cells can be primed by a short mild heat shock to survive a subsequent severe, otherwise lethal, heat stress. We wanted to assess the influence of transcriptional regulation during thermotolerance development in B. subtilis. We prepared total RNA of four differently treated samples of B. subtilis cells. For this purpose, exponential B. subtilis cells grown at 37 Â°C were divided and exposed for additional 15 min to temperatures of 37 Â°C, 48 Â°C, 53 Â°C or 15 min 48 Â°C followed by 15 min at 53 Â°C. Using four different merged dye swap experiments of red or green labelled cDNA of the above described RNA preparations, we compared and analyzed the transcriptional change of the conditions 48 Â°C vs. 53/48 Â°C (array 1), 37 Â°C vs. 53 Â°C (array 2), 37 Â°C vs. 48 Â°C (array 3) and especially 37/53Â°C vs. 53/48 Â°C (array 4)

ORGANISM(S): Bacillus subtilis

SUBMITTER: KÃ¼rsad Turgay

PROVIDER: E-GEOD-45972 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Thermotolerance in B. subtilis

Project description:During thermotolerance development, B. subtilis cells can be primed by a short mild heat shock to survive a subsequent severe, otherwise lethal, heat stress. We wanted to assess the influence of transcriptional regulation during thermotolerance development in B. subtilis. We prepared total RNA of four differently treated samples of B. subtilis cells. For this purpose, exponential B. subtilis cells grown at 37 °C were divided and exposed for additional 15 min to temperatures of 37 °C, 48 °C, 53 °C or 15 min 48 °C followed by 15 min at 53 °C.

2014-05-01 | GSE45972 | GEO

The role of (p)ppGpp in the heat shock response in B. subtilis

Project description:Bacterial and eukaryotic cells survive an otherwise lethal heat shock once they are primed by a mild pre-heat shock. Using B. subtilis as a model organism, we investigated transcriptional changes during thermotolerance development at 37 °C, 48 °C, 53 °C and 48/53 °C and observed a comprehensive upregulation of genes of the general and heat shock response and the downregulation of many translation-related genes. In addition, strains lacking (p)ppGpp (ppGpp0) or with elevated (p)ppGpp levels (ΔrelA) were analyzed at 37 °C and 48 °C, revealing broad and pleiotropic transcripional changes mediated by (p)ppGpp in the ΔrelA mutant. In addition, our data suggests a contribution of the (p)ppGpp-mediated stringent response in the heat-mediated transcripional down-regulation of ribosomal genes and additional processes.

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Project description:Transcriptional response of Bacillus subtilis KS002 to targocil Strain KS002 (Bacillus subtilis PY79 M-NM-^TtagGHBs::cat, amyE::Phyperspank tarGHSa spc) is a targocil sensitive B. subtilis strain, with TarGH from Staphylococcus aureus as the only WTA exporter, IPTG dependent (Schirner, Stone and Walker, ACS Chem Bio 2011). Strain KS002 was treated with or without targocil for 30 min. Each experiment was conducted three times using three independent total RNA preparations (biological triplicates). For each paried comparison, one sample was labeled with Alexa Fluor 555 and the other was with Alexa Fluor 647. For each comparison, one replicate was performed with dyeswap with the same RNA.

2014-02-01 | E-GEOD-54577 | biostudies-arrayexpress

Comparison of the transcription profile of B. subtilis clpX strain with B. subtilis clpX spx strain, reporting on Spx activity

Project description:In B. subtilis the unusual transcription factor Spx controls the thiol stress response regulon (Nakano et al 2003 PNAS 100:13603; Rochat et a. 2012 NAR 40:9751). Under normal growth conditions Spx is constantly degraded by the AAA+ protease complex ClpXP (Nakano et al 2001 MolMi 42:383). Comparing a B. subtilis clpX strain, where Spx levels are high, with a B. subtilis clpX spx double mutant strain which lacks Spx, will report on Spx dependent transcriptional control. We prepared total RNA of exponentially grown B. subtilis clpX and of B. subtilis clpX spx cells. Using a merged dye swap experiments of red or green labelled cDNA of the above described RNA preparations-

2014-09-01 | E-GEOD-50102 | biostudies-arrayexpress

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2011-06-17 | E-GEOD-30001 | biostudies-arrayexpress

A reversible light and genotype dependent acquired thermolerance response protects the potato plant from excessive temperature (microarray data set 2).

Project description:'Potato is particularly vulnerable to increased temperature, considered to be the most important uncontrollable factor affecting growth and yield. Here we describe an acquired thermotolerance response in potato, whereby treatment at a mildly elevated temperature (''acclimated'') primes the plant for more severe heat stress compared to control (''non-acclimated'') plants. We define the time course for acquiring thermotolerance and demonstrate that light is essential for the process. Physiological, transcriptomic and metabolomic approaches were employed to elucidate potential mechanisms that underpin the acquisition of heat tolerance and indicate a role for cell wall modification, auxin and ethylene signalling, and chromatin remodelling in acclimatory priming. This experiment identifies changes in transcript level were measured over a time course (0, 2, 6, 12 h) during the period of acquisition of thermotolerance following transfer from 18 °C (non-acclimated) to 25 °C (acclimated). Related experiment (E-MTAB-5857) looks to understand the mechanisms by which acclimation results in protection of leaves at extreme temperature; gene expression patterns were determined in leaves from acclimated and non-acclimated plants which were subsequently transferred to 40 °C and compared during a 48 h time course (2, 6, 12, 24 h).'

2018-04-04 | E-MTAB-5863 | biostudies-arrayexpress

The GntR family repressor YtrA responds to cell wall antibiotics

Project description:This SuperSeries is composed of the following subset Series: GSE30001: Bacillus subtilis 168 moenomycin stimulon GSE30002: Bacillus subtilis CU1065 ramoplanin stimulon Refer to individual Series

2011-06-17 | E-GEOD-30003 | biostudies-arrayexpress

Bacillus subtilis BJ3-2 transcriptome sequencing

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Reduction in membrane phosphatidylglycerol content leads to daptomycin resistance in Bacillus subtilis

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2011-06-16 | E-GEOD-30000 | biostudies-arrayexpress

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