Unknown,Transcriptomics,Genomics,Proteomics

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Gene is expression in 2 mutant alleles of the freezing-sensitive mutant sfr6 subjected to cold.


ABSTRACT: The sfr6-1 mutant of Arabidopsis has been shown to be defective in freezing tolerance and fails to express a number of cold-regulated genes to normal wild type levels. The aim of this experiment was to test whether two other mutant alleles, sfr6-2 and sfr6-3 showed similar defects in cold-inducible gene expression. Two experiments were performed. In each, one sfr6 mutant was cold-treated alongside its corresponding wild type.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Wouter Van Delm 

PROVIDER: E-GEOD-46084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Arabidopsis mediator complex subunits MED16, MED14, and MED2 regulate mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes.

Hemsley Piers A PA   Hurst Charlotte H CH   Kaliyadasa Ewon E   Lamb Rebecca R   Knight Marc R MR   De Cothi Elizabeth A EA   Steele John F JF   Knight Heather H  

The Plant cell 20140110 1


The Mediator16 (MED16; formerly termed SENSITIVE TO FREEZING6 [SFR6]) subunit of the plant Mediator transcriptional coactivator complex regulates cold-responsive gene expression in Arabidopsis thaliana, acting downstream of the C-repeat binding factor (CBF) transcription factors to recruit the core Mediator complex to cold-regulated genes. Here, we use loss-of-function mutants to show that RNA polymerase II recruitment to CBF-responsive cold-regulated genes requires MED16, MED2, and MED14 subuni  ...[more]

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