Project description:Transcript abundance in Escherichia coli O157:H7 was determined in the presence or absence of pulsed expression of the small RNA, AsxR. AsxR was cloned under the control the arabinose inducible promoter Para. Escherichia coli O157:H7 str. TUV93-0 with pAsxR or empty vector was cultured in MEM-HEPES media to an OD600 of 0.8 and 0.2% arabinose added. 10min after addition of arabinose 10ml of cells were harvested and and pellets resuspended in 1ml of Trizol and total RNA isolated. RNAs were labelled using the SuperScript Plus indirect cDNA labelling System. Triplicate control RNAs were pooled and hybridised to seperate AsxR test RNAs on three microarays. Arrays were hybridised using the Maui hybridisation platform and Scann using and Axon Autoloader Scanner. GenePix software was used to analyse images and GPR files were analysed using Genespring 7.3.1.
Project description:The objective of this study was to identify porcine genes which expression was affected by experimental infection with A. pleuropneumoniae within 24 hours after experimental challenge. Microarray experiments were conducted to reveal genes being significantly differentially expressed in infected versus non-infected lung tissue and in liver and lung lymph node tissue from three challenged versus two non-challenged animals. Keywords: Host response Three comparisons were done: - infected versus non-infected lung tissue sampled from three challenged pigs - liver tissue from three challenged versus two non-challenged animals - lung lymph node tissue from three challenged versus two non-challenged animals All experiments were conducted as common reference design.
Project description:gnp07_rilkit - rilkit-eqtl analysis (random pair design). To provide quantitative geneticists studying natural variation in our RIL populations with information and resources necessary to speed up QTL cloning and candidate genes identification. This articulates around the creation of two major resources and their integration into databases to make the information accessible to users: A. Whole transcriptome survey, B. Production of complementary material. Comparison of 164 Cvi/Col(8RV) Recombinant Inbred Lines (RILs) and 164 Bur/Col (20RV) RILs. 162 dye-swaps. Genotype and ecotype comparison.
Project description:gnp3_tri33-arabidoseed - eqtl analysis (random pair design) - WP3 : Biodiversity of seed traits : state of the art - Comparison of the developping seed transcriptome in 160 Bay0/Sha Recombinant Inbred Lines (RILs) at 10 days after pollinisation. Keywords: genotype and ecotype comparison 80 dye-swap - CATMA arrays
Project description:This SuperSeries is composed of the following subset Series: GSE24692: chip in silencing suppressor plants or in mirna plants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE24693: gene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE26739: Expression profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing Refer to individual Series
Project description:gen107_ptgs - gene profiling in silencing suppressor plants or in mirna mutants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - HcPro, P15, P19, CHS-RNAi (control), were grown on MS solid medium, and tissues were harvested at different developmental stages. dcl1-9, hen1-1 and La-er (control) were grown on MS solid medium, and tissues harvested at different developmental stages. Keywords: gene knock in (transgenic),gene knock out 60 dye-swap - CATMA arrays
Project description:gen107_ptgs - gene profiling in silencing suppressor plants or in mirna mutants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - HcPro, P15, P19, CHS-RNAi (control), were grown on MS solid medium, and tissues were harvested at different developmental stages. dcl1-9, hen1-1 and La-er (control) were grown on MS solid medium, and tissues harvested at different developmental stages. Keywords: gene knock in (transgenic),gene knock out 40 dye-swap - Chromochip Arabidopsis thaliana 21.7K CHROMO4_1
Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out 20 dye-swap pairs - CATMA arrays: 40 arrays
Project description:rs10-04_mad - comparison of transcriptomes in mad mutants - What gene sets are differentially expressed in mad mutants? - Seeds of GFP171.1 (parental line), mad1, mad2, mad3, mad6 and dcl1-12 were sterilized and germinated on Murashige/Skoog medium with 0.9% agar. Plates were stratified at 4C in the dark for 4 days. The plates were then transferred to a growth cabinet at 21C under a 16h light/8h darkness light regime, and the seedlings were harvested 18 days after transfer to the growth cabinet. 10 dye-swap - genotype comparaison