Widespread Transcription beyond mRNA 3’ Ends Yields Abundant Regulatory RNAs
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ABSTRACT: Through RNA-seq analyses of nascent transcripts, we found large numbers of RNA transcripts that extend beyond the 3’ ends of protein-coding genes; we refer to these extended transcripts as geRNAs. These findings demonstrate that transcription of most human protein-coding genes does not terminate within close proximity to poly(A) signals; rather, it terminates at sites far beyond these signals (up to 50 kb). Examination of different RNA species in HelaS3 cells by strand-specific RNA-seq. The 4sU-labeled RNA was isolated by using organomercurial affinity matrix.
ORGANISM(S): Homo sapiens
SUBMITTER: Shenglin Huang
PROVIDER: E-GEOD-46228 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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