Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from C. elegans during L3 and L3-lethargus


ABSTRACT: To identify genes differentially expressed during L3 lethargus, we collected RNA during the third larval stage (L3) lethargus period, 37 hours after feeding developmentally-arrested L1 animals. Animals in lethargus were identified based on quiescence of locomotion and feeding. Additional time point for RNA collection was in the mid-L3 stage, 32 hours after feeding developmentally-arrested L1 animals. These samples were interrogated with the Affymetrix C. elegans Genome Array. A total of 153 gene transcripts were up regulated, and 48 gene transcripts were down regulated, during the L3 lethargus period compared to the L3 stage (false discovery rate (FDR) < 0.05). There were 2 groups and 3x replication for each group, for 6 total samples. The groups were (1) L3 and (2) L3-lethargus. We compared L3-lethargus vs L3 using R/maanova. The permutation based p-values for each test were significant for FDRM-bM-^IM-$5%.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Keith Shockley 

PROVIDER: E-GEOD-46288 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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