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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq reveals changes in sphingolipid metabolism upon transformation

ABSTRACT: We sequenced mRNA from three independent pairs of mock and c-SRC transformed mouse embryonic fibroblasts and examined changes in expression of sphingolipid metabolism genes Three independent cultures of NIH3T3 MEFs were retrovirally transduced with either pBabe-Puro, og pBabe-Puro containing c-srcY527F. After a 7 day of selection in puromycine the three vector and three src transduced pools were propagated for RNA sequencing.

ORGANISM(S): Mus musculus

SUBMITTER: Mikkel Rohde

PROVIDER: E-GEOD-46340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transformation-associated changes in sphingolipid metabolism sensitize cells to lysosomal cell death induced by inhibitors of acid sphingomyelinase.

Petersen Nikolaj H T NH Olsen Ole D OD Groth-Pedersen Line L Ellegaard Anne-Marie AM Bilgin Mesut M Redmer Susanne S Ostenfeld Marie S MS Ulanet Danielle D Dovmark Tobias H TH Lønborg Andreas A Vindeløv Signe D SD Hanahan Douglas D Arenz Christoph C Ejsing Christer S CS Kirkegaard Thomas T Rohde Mikkel M Nylandsted Jesper J Jäättelä Marja M

Cancer cell 20130901 3

Lysosomal membrane permeabilization and subsequent cell death may prove useful in cancer treatment, provided that cancer cell lysosomes can be specifically targeted. Here, we identify acid sphingomyelinase (ASM) inhibition as a selective means to destabilize cancer cell lysosomes. Lysosome-destabilizing experimental anticancer agent siramesine inhibits ASM by interfering with the binding of ASM to its essential lysosomal cofactor, bis(monoacylglycero)phosphate. Like siramesine, several clinicall ...[more]

PMID: 24029234

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