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Transcription profiling of Arabidopsis seedlings exposed to ANep1 Treatment

ABSTRACT: In order to study the Nep1 responsive genes in Arabidopsis, experiments were performed with the Affymetrix GeneChip Arabidopsis ATH1 Genome Array (Santa Clara, CA; Cat # 900385). Experiment Overall Design: Seven-day old Arabidopsis seedlings were exposed to Nep1 (10 ug/mL) treatment or using sterilized, deionized H2O as a control for 30 min. Two independent biological replications were conducted using two independent samples and two microarrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hanhong Bae

PROVIDER: E-GEOD-4638 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Necrosis- and ethylene-inducing peptide from Fusarium oxysporum induces a complex cascade of transcripts associated with signal transduction and cell death in Arabidopsis.

Bae Hanhong H Kim Moon S MS Sicher Richard C RC Bae Hyeun-Jong HJ Bailey Bryan A BA

Plant physiology 20060512 3

Treatment of Arabidopsis (Arabidopsis thaliana) with a necrosis- and ethylene-inducing peptide (Nep1) from Fusarium oxysporum inhibited both root and cotyledon growth and triggered cell death, thereby generating necrotic spots. Nep1-like proteins are produced by divergent microbes, many of which are plant pathogens. Nep1 in the plant was localized to the cell wall and cytosol based on immunolocalization results. The ratio of chlorophyll a fluorescence (F685 nm/F730 nm) significantly decreased af ...[more]

PMID: 16698904

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