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Comparison between wild type E. coli and adhE (alcohol dehydrogenase) mutants, grown under anaerobic condition

ABSTRACT: To characterize anaerobic stress-induced expression in adhE mutants, the microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants. Under anaerobic growth condition in glucose-containing complex medium, the wild type strains 1 and 2 grew well whereas the mutant strains 3 and 4 experienced anaerobic stress and grew after 24 hours and 48 hours, respectively. For each of the four strains, RNA sampling was done after six hours of growth under anaerobic condition. Only one replicate was obtained for each strain.

ORGANISM(S): Escherichia coli BL21(DE3)

SUBMITTER: Seungwoo Hwang

PROVIDER: E-GEOD-46455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase.

Kim Hyun Ju HJ Jeong Haeyoung H Hwang Seungwoo S Lee Moo-Seung MS Lee Yong-Jik YJ Lee Dong-Woo DW Lee Sang Jun SJ

Frontiers in microbiology 20140909

Microbial adaptations often occur via genomic mutations under adverse environmental conditions. This study used Escherichia coli ΔadhE cells as a model system to investigate adaptation to anaerobic conditions, which we then compared with the adaptive mechanisms of two closely related E. coli strains, K-12 and B. In contrast to K-12 ΔadhE cells, the E. coli B ΔadhE cells exhibited significantly delayed adaptive growth under anaerobic conditions. Adaptation by the K-12 and B strains mainly employe ...[more]

PMID: 25250024

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