Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from wildtype and mec-3 mutant PVD/OLL sensory neurons in C. elegans


ABSTRACT: Nociceptive neurons develop a complex dendritic arbor to sense noxious stimuli, which enables animals to react to environmental insults and perform self-protective behaviours. The genetic programs controlling neuronal dendritic morphogenesis are poorly understood. In C. elegans, the PVD sensory neuron generates a complex dendritic arbor that envelops the body of the animal. This nociceptive neuron enables study of dendrite formation in vivo. MEC-3 is a transcription factor expressed in the PVD neuron (Chatzigeorgiou et al., 2010; Li et al., 2011; Way and Chalfie, 1989). mec- 3 mutant PVD neurons fail to elaborate lateral branches and show defective function (Smith et al., 2010; Tsalik et al., 2003) (Husson et al., 2012). We used tiling arrays to identify genes regulated by the mec-3 transcription factor in PVD sensory neurons. We employ the mRNA-tagging method to isolate poly(A) RNA from the PVD and OLL neurons in wildtype and mec-3 mutants (3 replicates each) by expressing a 3X FLAG-tagged poly(A) binding protein PAB-1 in PVD and OLL under control of the ser-2prom3B promoter. The enriched poly(A) RNA is amplified using the NuGEN WT-Pico amplification system and applied to Affymetrix C. elegans tiling microarrays. Whole animal reference samples were obtained by isolating total RNA at the L2/L3 stages in wildtype and mec-3 mutants (2 replicates each) and processed as above.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: William Spencer 

PROVIDER: E-GEOD-46530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2013-05-02 | GSE46530 | GEO
| PRJEB7907 | ENA
2009-04-22 | E-GEOD-15758 | biostudies-arrayexpress
| PRJNA433383 | ENA
2020-10-28 | PXD019882 | Pride
| PRJEB53879 | ENA
| PRJNA721984 | ENA
| PRJNA729106 | ENA
2019-08-01 | GSE135132 | GEO
2020-07-21 | PXD015969 | Pride