Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genetic reconstruction of a functional transcriptional regulatory network

ABSTRACT: Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors. Data values are X scores and corresponding p-values derived using an error model. We profiled transcriptional responses upon the individual deletion of more than 260 TFs. We used a directed-weighted graph modelling approach and regulatory epistasis analysis to identify indirect regulatory relationships, and thus constructed a refined, functional transcriptional regulatory network.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Killion

PROVIDER: E-GEOD-4654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genetic reconstruction of a functional transcriptional regulatory network.

Hu Zhanzhi Z Killion Patrick J PJ Iyer Vishwanath R VR

Nature genetics 20070408 5

Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph m ...[more]

PMID: 17417638

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Dataset Information

Genetic reconstruction of a functional transcriptional regulatory network

Publications

Genetic reconstruction of a functional transcriptional regulatory network.

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