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MicroRNA Target Site Identification by Integrating Sequence and Binding Information

ABSTRACT: High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flexible computational framework that integrates sequence with cross-linking features reliably identifies the microRNA family involved in each binding event, considerably outperforms sequence-only approaches, and quantifies the prevalence of noncanonical binding modes. Ago2 (Argonaute 2) PAR-CLIP and RNA deep sequencing of Epstein-Barr virus B95.8-infected Lymphoblastoid Cell Lines (LCLs)

ORGANISM(S): Homo sapiens

SUBMITTER: Neelanjan Mukherjee

PROVIDER: E-GEOD-46611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The viral and cellular microRNA targetome in lymphoblastoid cell lines

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microRNA Targetome Analysis of Latently KSHV-infected Primary Effusion Lymphoma Cell lines Using PAR-CLIP

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2011-11-16 | E-GEOD-32113 | biostudies-arrayexpress

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