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Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

ABSTRACT: To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer Postnatal day 6 testes (2) of pups sired by males treated with saline (n=5) or BEP (n=5) for a 9 weeks followed by a 9-week recovery period

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jennifer Maselli

PROVIDER: E-GEOD-46910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

Project description:To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer

2013-05-15 | GSE46910 | GEO

Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

2014-03-13 | GSE55842 | GEO

Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

Project description:8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating. qPCR gene expression profiling for 84 stem cell transcription factors. Total RNA extracted from 25-35 embryos at the eight-cell stage sired by control or BEP-treated males after a 9-wk recovery period (2 females mated per male, n= 4 males/treatment).

2014-03-13 | E-GEOD-55842 | biostudies-arrayexpress

Expression data from neonatal rat ovaries following LPS exposure

Project description:Inflammatory processes play an important role in regulation of ovarian development, in particularly early in life. Neonatal immune challenge by administration of lipopolysaccharide (LPS) has been previously demonstrated to result in diminished ovarian reserve and altered reproductive lifespan. In this study we aimed to characterise the cellular mechanisms that may underpin impaired ovulatory capacity and reduced oocyte development, induced by LPS treatment. Rat pups were administered with LPS or saline on postnatal days 3 and 5. Ovaries were obtained on postnatal day 7 to examine the effect of LPS administration on the ovarian transcriptome. Rat pups were administered intraperitoneally either 0.05mg/kg of LPS (Salmonella Enteritidis) or an equivalent volume of non-pyrogenic saline on postnatal days (PNDs) 3 and 5, and ovaries were obtained on PND 7 for RNA extraction and hybridization on an Agilent-028279 SurePrint G3 Rat GE 8x60K Array platform.

2013-04-27 | E-GEOD-46436 | biostudies-arrayexpress

Rattus norvegicus

Project description:Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

| PRJNA203020 | ENA

Effect of chronic glucocorticoid (GC) treatment on neonatal mouse whole cerebellum and whole lung

Project description:Dexamethasone (1ug/g, Dex) or vehicle (saline) injected into postnatal days 0-7 (P0 to P7) of C57BL6J mouse pups every day. Whole organ were harvested at P7.

2013-09-26 | GSE51213 | GEO

Whole brain expression data from postnatal day 7-aged mice exposed to ethanol at postnatal day 7

Project description:The developing brain is particularly sensitive to ethanol during the brain growth spurt or synaptogenesis (third human trimester equivalent). This has been shown to lead to abnormal brain development and behavioural changes in the adult mouse that are relevant to those seen in humans with fetal alcohol spectrum disorders (FASD). We evaluated the acute (4h post-treatment) gene expression changes that occur in the brain due to ethanol exposure during synaptogenesis (postnatal day 7). We used microarray analyses to evaluate the changes in brain gene expression at postnatal day 7 that occur due to ethanol treatment at postnatal day 7 (synaptogenesis). To generate samples, C57BL/6J pups were injected with ethanol (experimental) or saline (control) at postnatal day 7 (2 x 2.5 g/kg at 0h and 2h). Pups were sacrificed 4 hours following the initial injection. Total RNA was extracted from whole brain tissue and RNA from three male mice from three different litters were pooled as one biological replicate. Each male ethanol-treated mouse represented in a sample was matched by a control littermate present in a control sample. This study consists of two experimental (ethanol-treated) biological replicates and four control (saline vehicle-treated) replicates (total mice used was n=18).

2014-07-18 | E-GEOD-59512 | biostudies-arrayexpress

Expression data from gonads of Meioc+/+ and Meioc-/-mice

Project description:Meiosis is a specialized division that is specifically engaged in germ cells. This program is finely and dichotomically regulated: germ cells enter meiosis at fetal life in ovaries (13.5 dpc) and only at post-natal life in testes (8 dpp). We used microarrays to determine the gene expression modifications in Meioc mutant gonads displaying early meiotic defects. 14.5 dpc ovaries and 8 dpp testes from Meioc+/+ and Meioc-/- mice (NMRI) were dissected and processed for RNA extraction and hybridization on Affymetrix microarrays. Two pools were analysed for each genotype and each sex. We sought to constitutes pairs (A and B) of wild type and knock-out pools from embryos and pups that were homogenous for developmental stages and from matched littermates. 10 ovaries from 5 embryos composed each pool for females and 4 testes from 4 pups composed each pool for males.

2015-11-19 | E-GEOD-66880 | biostudies-arrayexpress

brain expression data from adult mice exposed to ethanol at postnatal day 4 and 7

Project description:The developing brain is particularly sensitive to ethanol during the brain growth spurt or synaptogenesis (third human trimester equivalent). This has been shown to lead to abnormal brain development and behavioural changes in the adult mouse that are relevant to those seen in humans with fetal alcohol spectrum disorders (FASD). We evaluated the long-term (postnatal day 60 young adult) gene expression changes that occur in the brain due to ethanol exposure during synaptogenesis. We used microarray analyses to evaluate the changes in brain gene expression at postnatal day 60 that occur due to ethanol treatment at postnatal days 4 and 7 (synaptogenesis). To generate samples, C57BL/6J pups were injected with ethanol (experimental) or saline (control) at postnatal days 4 and 7. Pups were weaned at postnatal day 25 and sacrificed at postnatal day 60. Total RNA was extracted from whole brain tissue and RNA from three male mice from three different litters were pooled as one biological replicate. Each male ethanol-treated mouse represented in a sample was matched by a control littermate present in a control sample. This study consists of two biological replicates for each experimental group (total mice used was n=12).

2011-12-20 | E-GEOD-34549 | biostudies-arrayexpress

Effect of chronic glucocorticoid (GC) treatment on neonatal mouse whole cerebellum and whole lung

Project description:Dexamethasone (1ug/g, Dex) or vehicle (saline) injected into postnatal days 0-7 (P0 to P7) of C57BL6J mouse pups every day. Whole organ were harvested at P7. Biological replicates (n = 4) per condition: whole cerebellum or whole lung, treated with dexamethasone or vehicle. This microarray experiment was performed concurrently with the study described in PMID:19164857. The culture and experiment designs in PMID:19164857 are identical to those in this microarray experiment. KEYWORDS: cell type comparison, treatment comparison.

2013-09-26 | E-GEOD-51213 | biostudies-arrayexpress

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