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Streptococcus suis 05ZY: control vs contact with hBMEC 1 h

ABSTRACT: The interactions of Streptocococcus suis (S.suis) with human microvascular endothelial cells (hBMEC) are important process for S.suis passage across human blood brain barrier (BBB). S.suis has evolved precise mechanisms to alter gene expression depending on the distinct challenges posed by particular disease sites. Herein, a whole-genome DNA microarray was used to investigate the change of gene expression profile of S.suis after contact with hBMEC 1 h. comparison of RNA isolated from hBMEC-associated S.suis with RNA derived from control bacteria revealed significant differential changes for 219 S.suis genes including 131 up-regulated genes and 88 down-regulated genes at 1 h post infection. A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, Two-condition experiment, control vs contact with hBMEC 1 h, four replicates (two biological replicates, two technical replicates) at each condition.

ORGANISM(S): Streptococcus suis 05ZYH33

SUBMITTER: Huaijie Hao

PROVIDER: E-GEOD-46961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Streptococcus suis 05ZY: control vs contact with hBMEC 3 h

Project description:The interactions of Streptocococcus suis (S.suis) with human microvascular endothelial cells (hBMEC) are important process for S.suis passage across human blood brain barrier (BBB). S.suis has evolved precise mechanisms to alter gene expression depending on the distinct challenges posed by particular disease sites. Herein, a whole-genome DNA microarray was used to investigate the change of gene expression profile of S.suis after contact with hBMEC 3 h. comparison of RNA isolated from hBMEC-associated S.suis with RNA derived from control bacteria revealed significant differential changes for 175 S.suis genes including 123 up-regulated genes and 52 down-regulated genes at 3 h post infection. A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, Two-condition experiment, control vs contact with hBMEC 3 h, four replicates (two biological replicates, two technical replicates) at each condition.

2015-12-31 | E-GEOD-46962 | biostudies-arrayexpress

Streptococcus pneumoniae cDNA microarray data used to characterize the transcriptional responses occurring during initial contact between pneumococci and a human pleural mesothelial cell line (PMC) in vitro.

Project description:Streptococcus pneumoniae (Spn) is a major causative organism of empyema, an inflammatory condition occurring in the pleural sac. In this study, we used Spn cDNA microarrays to characterize the transcriptional responses occurring during initial contact between Spn and a human pleural mesothelial cell line (PMC) in vitro

2016-03-30 | E-MTAB-3931 | biostudies-arrayexpress

Transcriptional profiling of a flpS knock-out strain of Steptococcus suis strain 10

Project description:Transcriptional profiling of a S. suis 10flpS::spcR mutant strain in which the flpS gene has been disrupted by an insertion of a spectinomycin resistance cassette (spcR). We compared the expression profile of S. suis strain 10 with that of S. suis strain 10flpS::spcR during exponential and stationary growth of the bacterial cells.

2016-10-11 | E-MTAB-3997 | biostudies-arrayexpress

Transcriptome comparison of a Streptococcus suis wildytpe and a ccpA knock-out strain in stationary growth phase

Project description:Transcriptome comparison of a Streptococcus suis wildytpe and a ccpA knock-out strain in stationary growth phase

2014-01-13 | E-MEXP-3832 | biostudies-arrayexpress

Transcriptome comparison of Streptococcus suis wildytpe and a ccpA knock-out strain in exponential growth phase

Project description:Transcriptome comparison of Streptococcus suis wildytpe and a ccpA knock-out strain in exponential growth phase.

2010-12-31 | E-MEXP-2946 | biostudies-arrayexpress

ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment

Project description:Transcriptome comparison of Streptococcus suis wildtype and argR knock-out strain grown to exponential or early-stationary phase, respectively.

2010-10-17 | E-MEXP-2945 | biostudies-arrayexpress

The differentially expressed genes of A. ferrooxidans ATCC 23270 at different time after introduced fluoride

Project description:The deleterious fluoride ions, which released from fluoride in uranium during its bioleaching, strongly influenced microbial growth, energy intake, enzyme activity and relative metabolism. Whole-genome microarrays were used to obtain a comprehensive description of the molecular response by A. ferrooxidans ATCC 23270 at 4.8 mM fluoride stress. The experimental group was introduced 4mM fluoride at the mid-log phase. RNA from control samples were compared to the experimental samples taken at 10, 30, 60, 120 and 240 min after fluoride added. It was used to analyze the genome-wide expression profiling at different time after fluoride was introduced.

2016-07-03 | E-GEOD-76450 | biostudies-arrayexpress

Genome wide analysis of C57BL/6 mice and C57BL/6 TLR2-KO mice infected with European strain (P1/7) of Streptococcus suis

Project description:Streptococcus suis is an emerging zoonotic agent causing meningitis and septicemia. Outbreaks in humans in China with atypical cases of streptococcal toxic shock-like syndrome have been described to be caused by a clonal epidemic S. suis strain characterized as sequence type (ST) 7 by multilocus sequence typing, different from the classical ST1 usually isolated in Europe. Previous in vitro studies showed that Toll-like receptor (TLR) 2 plays a major role in S. suis ST1 interactions with host cells. In the present study, the in vivo role of TLR2 in systemic infections caused by S. suis ST1 or ST7 strains using TLR2 deficient (TLR2-/-) mice was evaluated. TLR2-mediated recognition significantly contributes to the acute disease caused by the highly virulent S. suis ST1 strain, since the TLR2-/- mice remained unaffected when compared to wild type (WT) mice. The lack of mortality could not be associated with a lower bacterial burden; however, a significant decrease in the induction of pro-inflammatory mediators, as evaluated by microarray, real-time PCR and protein assays, was observed. On the other hand, TLR2-/- mice infected with the epidemic ST7 strain presented no significant differences regarding survival and expression of pro-inflammatory mediators when compared to the WT mice. Together, these results show a TLR2-independent host innate immune response to S. suis that depends on the strain. Total RNA obtained from spleen of C57BL/6 mice or C57BL/6 TLR2-KO mice infected with Streptococcus suis strain P1/7. Four replicates in both groups.

2013-06-01 | E-GEOD-45861 | biostudies-arrayexpress

Genome wide analysis of C57BL-6 mice and C57BL/6 TLR2-KO mice infected with Chinese strain (SC84) of Streptococcus suis

2013-06-01 | E-GEOD-45862 | biostudies-arrayexpress

Genome wide analysis of C57BL-6 mice infected with North-American strain (89-1591) of Streptococcus suis

Project description:Streptococcus suis is a major swine pathogen that can be transmitted to humans causing severe symptoms. A large human outbreak was described in China, where approximately 25% out of 215 infected humans developed an unusual streptococcal toxic shock-like syndrome (STSLS). Albeit increased expression of inflammatory mediators following infection by the Chinese S. suis strain was suggested as responsible for STSLS case severity, the mechanisms involved are still poorly understood. In this study, we investigated the host innate immune response to infection by either one of 3 strains of S. suis: 89-1591 (Canadian, intermediate virulence), P1/7 (European, high virulence), and SC84 (Chinese, epidemic strain). Using Illumina microarray and validating those results with qPCR and Luminex assay, infected mice showed elevated expression of mainly pro-inflammatory chemokine and cytokine genes. Generally, pro-inflammatory genes were expressed at a higher level in mice infected with S. suis strain SC84 > P1/7 > 89-1591. Interestingly, IFNM-NM-3 was expressed at much higher levels only in mice infected with the S. suis strain SC84, which could potentially explain some of the STSLS symptoms. IFNM-NM-3-KO mice infected with SC84 showed better survival than WT mice while no differences was seen in mice infected with highly virulent P1/7 strain. Overall, our results show an important role of IFNM-NM-3 in S. suis infections and might explain in part the increased virulence of SC84 responsible for a recent outbreak in China. Total RNA obtained from spleen of C57BL/6 mice infected with Streptococcus suis strain 89-1591 or mock-infected with THB. Four replicates in both groups.

2013-03-27 | E-GEOD-41520 | biostudies-arrayexpress

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