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Potential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca)

ABSTRACT: The goal is to examine the molecular aspects underpinning somatic embryogenesis (SE) within shoot tissues of adult white spruce trees by conducting transcriptome-wide expression profiling of shoot explants taken from responsive and nonresponsive genotypes. One-color analysis of vegetative buds in somatic embryogenesis responsive and nonresponsive genotypes, before and after 7 days of SE induction. There are 5 biological replicates for each of the 4 combinations of genotype/time of induction for a total of 20 slides. Each replicate is comprised of a pool of 6 buds.

ORGANISM(S): Picea glauca

SUBMITTER: SM-CM-)bastien Caron

PROVIDER: E-GEOD-46977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Potential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca).

Rutledge Robert G RG Stewart Don D Caron Sébastien S Overton Cathy C Boyle Bryan B MacKay John J Klimaszewska Krystyna K

BMC plant biology 20130812

<h4>Background</h4>Among the many commercial opportunities afforded by somatic embryogenesis (SE), it is the ability to clonally propagate individual plants with rare or elite traits that has some of the most significant implications. This is particularly true for many long-lived species, such as conifers, but whose long generation times pose substantive challenges, including increased recalcitrance for SE as plants age. Identification of a clonal line of somatic embryo-derived trees whose shoot ...[more]

PMID: 23937238

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