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Transcriptome comparison of murine wild-type and dumbo retinas at P15

ABSTRACT: Hmx1 is a transcription factor expressed in the developing eye and ear and in some other parts of the nervous system. Dumbo mice are carrying the Hmx1 p.Q64X loss-of-function mutation (Munroe et al., 2009. BMC Developmental Biology). Transcriptomic analyses of this mouse model allows to decipher biological pathways under the control of Hmx1. In our study, we used it to better understand the role of Hmx1 in the retina and to identify several of its target genes. Wild-type and dumbo mice were maintained in a 12-hours light/12-hours dark cycle with free access to food and water. Animals were sacrified at P15 for retina isolation and RNA extraction.

ORGANISM(S): Mus musculus

SUBMITTER: Arnaud Boulling

PROVIDER: E-GEOD-47002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of HMX1 target genes: a predictive promoter model approach.

Boulling Arnaud A Wicht Linda L Schorderet Daniel F DF

Molecular vision 20130806

<h4>Purpose</h4>A homozygous mutation in the H6 family homeobox 1 (HMX1) gene is responsible for a new oculoauricular defect leading to eye and auricular developmental abnormalities as well as early retinal degeneration (MIM 612109). However, the HMX1 pathway remains poorly understood, and in the first approach to better understand the pathway's function, we sought to identify the target genes.<h4>Methods</h4>We developed a predictive promoter model (PPM) approach using a comparative transcripto ...[more]

PMID: 23946633

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