Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of spontaneously hypertensive and Sprague Dawley rat lung tissue from subjects exposed to sulfur dioxide and controls

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is diagnosed by airway obstruction and underlies a group of ailments such as bronchitis, emphysema and often asthma; however, rodent models do not resemble human pathology. Because genetically predisposed spontaneously hypertensive (SH) rats display phenotypes such as systemic inflammation, thrombosis, oxidative stress, and suppressed immune function, that are also apparent in COPD patients, we exposed SH and commonly used male Sprague Dawley (SD) to 0, 250, or 350 ppm sulfur dioxide (SO2), 5h/d for 4 consecutive days. Airways disease was characterized by pulmonary functional, pathological and molecular analysis. SO2 caused dose-dependent changes in breathing parameters in both strains with SH rats being slightly more affected than SD. Bronchoalveolar lavage fluid (BALF) total cells and neutrophilic inflammation were dose-dependent and significantly greater in SH than in SD rats. The recovery was incomplete 4-day following SO2 exposure in SH rats. Pulmonary protein leakage did not occur in either strain but lactate dehydrogenase and n-acetyl glucosaminidase activity was increased in BALF of SH rats. Lung pathology and morphometric evaluation of mucin production in the airways demonstrated significantly greater impact of SO2 in SH than in SD rats. Baseline differences in lung gene expression pattern suggested marked immune dysregulation, oxidative stress, and impairment cell signaling and fatty acid metabolism in SH rats. Gene expression pattern of SD and SH rats following SO2 exposure demonstrated greater effect on inflammation/immune markers, and oxidative stress. Thus, the SH rat may serve as a better and more susceptible rat strain to be used for experimental model of bronchitis which is relevant to human disease. Experiment Overall Design: Spontaneously hypertensive and Sprague Dawley rats were used in this experiment. Each strain of rat was treated with either SO2 or AIR (control). There were 4 biological replicates for each strain/treatment.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Urmila Kodavanti

PROVIDER: E-GEOD-4702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The spontaneously hypertensive rat: an experimental model of sulfur dioxide-induced airways disease.

Kodavanti Urmila P UP Schladweiler Mette C MC Ledbetter Allen D AD Ortuno Roselia Villalobos RV Suffia Marie M Evansky Paul P Richards Judy H JH Jaskot Richard H RH Thomas Ronald R Karoly Edward E Huang Yuh-Chin T YC Costa Daniel L DL Gilmour Peter S PS Pinkerton Kent E KE

Toxicological sciences : an official journal of the Society of Toxicology 20060823 1

Chronic obstructive pulmonary disease (COPD) is characterized by airway obstruction, inflammation, and mucus hypersecretion, features that are common in bronchitis, emphysema, and often asthma. However, current rodent models do not reflect this human disease. Because genetically predisposed spontaneously hypertensive (SH) rats display phenotypes such as systemic inflammation, hypercoagulation, oxidative stress, and suppressed immune function that are also apparent in COPD patients, we hypothesiz ...[more]

PMID: 16929007

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Dataset Information

Transcription profiling of spontaneously hypertensive and Sprague Dawley rat lung tissue from subjects exposed to sulfur dioxide and controls

Publications

The spontaneously hypertensive rat: an experimental model of sulfur dioxide-induced airways disease.

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