Allele specific analysis of the immunoglobulin heavy chain locus
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: sjoerd holwerda
PROVIDER: E-GEOD-47129 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Allelic exclusion of the immunoglobulin heavy chain locus is independent of its nuclear localization in mature B cells.
Holwerda Sjoerd J B SJ van de Werken Harmen J G HJ Ribeiro de Almeida Claudia C Bergen Ingrid M IM de Bruijn Marjolein J W MJ Verstegen Marjon J A M MJ Simonis Marieke M Splinter Erik E Wijchers Patrick J PJ Hendriks Rudi W RW de Laat Wouter W
Nucleic acids research 20130607 14
In developing B cells, the immunoglobulin heavy chain (IgH) locus is thought to move from repressive to permissive chromatin compartments to facilitate its scheduled rearrangement. In mature B cells, maintenance of allelic exclusion has been proposed to involve recruitment of the non-productive IgH allele to pericentromeric heterochromatin. Here, we used an allele-specific chromosome conformation capture combined with sequencing (4C-seq) approach to unambigously follow the individual IgH alleles ...[more]
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