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Expression data from visceral mesothelium (omentum) and parietal meosthelium

ABSTRACT: Mesothelia, which cover all coelomic organs and body cavities in vertebrates, perform diverse functions in embryonic and adult life. Yet, mesothelia are traditionally viewed as simple, uniform epithelia. We used microarrays to demonstrate distinct differences between visceral and parietal mesothelia, the most basic subdivision of this tissue type, in terms of gene expression. Visceral mesothelium was isolated from the omentum of adult Wt1-cre;Rosa26ReYFP mice. In parallel, parietal mesothelium was isolated by teasing the mesothelium from the underlying skeletal muscle of the body wall of these animals. RNA was then extracted from these native mesothelia samples and used for hybridization in a microarray experiment.

ORGANISM(S): Mus musculus

SUBMITTER: David Bader

PROVIDER: E-GEOD-47161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from visceral mesothelium (omentum) and parietal meosthelium

Project description:Mesothelia, which cover all coelomic organs and body cavities in vertebrates, perform diverse functions in embryonic and adult life. Yet, mesothelia are traditionally viewed as simple, uniform epithelia. We used microarrays to demonstrate distinct differences between visceral and parietal mesothelia, the most basic subdivision of this tissue type, in terms of gene expression.

2013-05-22 | GSE47161 | GEO

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Project description:Expression data from visceral mesothelium (omentum) and parietal meosthelium

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Project description:The gene expression profiles of murine visceral and parietal mesothelia were analyzed by microarray. Visceral and parietal mesothelia were isolated from the same adult Wt1-cre;Rosa26ReYFP reporter mice and analyzed. Whole tracscriptome expression analysis of total RNA was performed using Affymetrix mouse Gene 1.0 ST arrays.

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Project description:The mesothelium forms epithelial membranes that line the bodies cavities and surround the internal organs. Mesothelia widely contribute to organ homeostasis and regeneration, and their dysregulation can result in congenital anomalies of the viscera, ventral wall defects, and mesothelioma tumors. Nonetheless, the embryonic ontogeny and developmental regulation of mesothelium formation has remained uncharted. Here, we combine genetic lineage tracing, in toto live imaging, and single-cell transcriptomics in zebrafish to track mesothelial progenitor origins from the lateral plate mesoderm (LPM). Our single-cell analysis uncovers a post-gastrulation gene expression signature centered on hand2 that delineates distinct progenitor populations within the forming LPM. Combining gene expression analysis and imaging of transgenic reporter zebrafish embryos, we chart the origin of mesothelial progenitors to the lateral-most, hand2-expressing LPM and confirm evolutionary conservation in mouse. Our time-lapse imaging of transgenic hand2 reporter embryos captures zebrafish mesothelium formation, documenting the coordinated cell movements that form pericardium and visceral and parietal peritoneum. We establish that the primordial germ cells migrate associated with the forming mesothelium as ventral migration boundary. Functionally, hand2 mutants fail to close the ventral mesothelium due to perturbed migration of mesothelium progenitors. Analyzing mouse and human mesothelioma tumors hypothesized to emerge from transformed mesothelium, we find de novo expression of LPM-associated transcription factors, and in particular of Hand2, indicating the re-initiation of a developmental transcriptional program in mesothelioma. Taken together, our work outlines a genetic and developmental signature of mesothelial origins centered around Hand2, contributing to our understanding of mesothelial pathologies and mesothelioma.

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