Unknown,Transcriptomics,Genomics,Proteomics

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SALT-RESPONSIVE ERF1 expression profiling in rice roots


ABSTRACT: To identify genes that are regulated by SERF1, we performed expression profiling on roots of serf1 and wild-type plants under standard growth conditions. Plants were grown for 4 weeks at (day/night) 26/22°C, 75/70% relative humidity with a day-length of 12 h and a light intensity of 700 µmol m-2 s-1. Seeds of serf1 T-DNA lines and Nipponbare wild type were placed on hydroponic boxes with full-strength Yoshida medium (Yoshida et al., 1971) using styrofoam adaptors. For each genotype 3 biological replicates were separately isolated and measured.

ORGANISM(S): Oryza sativa

SUBMITTER: Jos Schippers 

PROVIDER: E-GEOD-47195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice.

Schmidt Romy R   Schippers Jos H M JH   Mieulet Delphine D   Watanabe Mutsumi M   Hoefgen Rainer R   Guiderdoni Emmanuel E   Mueller-Roeber Bernd B  

Molecular plant 20130917 2


Grain quality is an important agricultural trait that is mainly determined by grain size and composition. Here, we characterize the role of the rice transcription factor (TF) SALT-RESPONSIVE ERF1 (SERF1) during grain development. Through genome-wide expression profiling and chromatin immunoprecipitation, we found that SERF1 directly regulates RICE PROLAMIN-BOX BINDING FACTOR (RPBF), a TF that functions as a positive regulator of grain filling. Loss of SERF1 enhances RPBF expression resulting in  ...[more]

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