Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human aortic smooth muscle cells exposed to hypoxia


ABSTRACT: Systemic arterial smooth muscle cells are exposed to a broad range of oxygen concentrations under physiological conditions. Hypoxia can modulate the proliferative response of smooth muscle cells leading to speculation about its role in vasculogenesis, vascular remodelling and the pathogenesis of arterial disease. The effect of hypoxia has been inconsistent, however, with both enhanced proliferation and growth arrest reported. Nevertheless, these reports support an important effect of hypoxia on smooth muscle cell proliferation and, given its physiological and clinical relevance, this requires clarification. We posited that variation in O2 concentration, within the range that exists in vivo, may have different effects on the proliferation and survival of vascular smooth muscle cells. Experiment Overall Design: Human aortic smooth muscle cells (HASMC) were propagated to passage 6 in SMGM-2 medium reached 80% confluence, the media was changed and the cells were incubated for a further 16 hrs or 48 hrs under either normoxic or hypoxic conditions (1% and 3%O2 ).

ORGANISM(S): Homo sapiens

SUBMITTER: Sara Arab 

PROVIDER: E-GEOD-4725 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Oxygen regulation of arterial smooth muscle cell proliferation and survival.

Ray Julie Basu JB   Arab Sara S   Deng Yupu Y   Liu Peter P   Penn Linda L   Courtman David W DW   Ward Michael E ME  

American journal of physiology. Heart and circulatory physiology 20071130 2


The purpose of this study was to determine if hypoxia elicits different proliferative and apoptotic responses in systemic arterial smooth muscle cells incubated under conditions that do or do not result in cellular ATP depletion and whether these effects are relevant to vascular remodeling in vivo. Gene expression profiling was used to identify potential regulatory pathways. In human aortic smooth muscle cells (HASMCs) incubated at 3% O(2), proliferation and progression through the G1/S interpha  ...[more]

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