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The impact of miR-542-3p on gene expression profile in U2OS cells

ABSTRACT: Analysis of gene expression change in U2OS cells expression synthetic miR-542-3p mimics. Forty-eight hours after transfection with negative miRNA mimics or miR-542-3p mimics, U2OS cells were subjected to RNA isolation.

ORGANISM(S): Homo sapiens

SUBMITTER: Yemin Wang

PROVIDER: E-GEOD-47363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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p53 is positively regulated by miR-542-3p.

Wang Yemin Y Huang Jen-Wei JW Castella Maria M Huntsman David George DG Taniguchi Toshiyasu T

Cancer research 20140424 12

The tumor suppressor p53 and miRNAs are linked through a complex network. Several miRNAs modulate p53 expression, while p53 regulates the transcription and/or biogenesis of several other miRNAs. Here, we report the development of a cell-based assay used with a library of human miRNA mimics in a high-throughput screen for miRNAs that modulate p53 expression. Overexpression of miRNA (miR)-542-3p in cancer cells elevated p53 expression, stimulated the expression of p53 targets, and inhibited cell p ...[more]

PMID: 24762395

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