Unknown,Transcriptomics,Genomics,Proteomics

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Regulation of Gene Expression by Connective Tissue Growth Factor (CTGF) in Mesenchymal Stromal Cells (MSCs)


ABSTRACT: mRNA from bone marrow-derived MSCs stably expressing CTGF-specific shRNA (or empty vector control) was analyzed for differential gene expression. Significant differences were found in cell proliferation-related genes, especially genes related to the M phase of the cell cycle, which were down-regulated in CTGF-knockdown-MSCs compared to control MSCs. Bone marrow-derived MSCs were stably transduced with lentivirus expressing CTGF-specific shRNA or an empty vector control. After antibiotic selection, total RNA was amplified and hybridized to Illumina HT12 version 3 human whole-genome arrays (Illumina, San Diego, CA).

ORGANISM(S): Homo sapiens

SUBMITTER: Wencai Ma 

PROVIDER: E-GEOD-47575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Connective tissue growth factor regulates adipocyte differentiation of mesenchymal stromal cells and facilitates leukemia bone marrow engraftment.

Battula V Lokesh VL   Chen Ye Y   Cabreira Maria da Graca Mda G   Ruvolo Vivian V   Wang Zhiqiang Z   Ma Wencai W   Konoplev Sergej S   Shpall Elizabeth E   Lyons Karen K   Strunk Dirk D   Bueso-Ramos Carlos C   Davis Richard Eric RE   Konopleva Marina M   Andreeff Michael M  

Blood 20130605 3


Mesenchymal stromal cells (MSCs) are a major component of the leukemia bone marrow (BM) microenvironment. Connective tissue growth factor (CTGF) is highly expressed in MSCs, but its role in the BM stroma is unknown. Therefore, we knocked down (KD) CTGF expression in human BM-derived MSCs by CTGF short hairpin RNA. CTGF KD MSCs exhibited fivefold lower proliferation compared with control MSCs and had markedly fewer S-phase cells. CTGF KD MSCs differentiated into adipocytes at a sixfold higher rat  ...[more]

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