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DNA methylation profiles across different brain regions in healthy mice (control samples)

ABSTRACT: Genome wide DNA methylation profiling of normal and disease samples from different mice models. A custom Illumina Golden Gate DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 800 CpGs. Bisulphite converted DNA from the 7 control samples were hybridised to the Illumina custom golden gate DNA methylation array.

ORGANISM(S): Mus musculus

SUBMITTER: Antonio Gómez

PROVIDER: E-GEOD-47576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation profiles across different brain regions in APP/PSEN1 mice

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2015-12-05 | E-GEOD-42745 | biostudies-arrayexpress

DNA methylation profiles across different brain regions in healthy mice

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DNA methylation profiles across different brain regions in APP/PSEN1 mice

2015-12-05 | GSE42745 | GEO

DNA methylation profiles across different brain regions in healthy mice (control samples)

2014-06-01 | GSE47576 | GEO

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caArray_laird-00051: TCGA Analysis of DNA Methylation for GBM Using Illumina Golden Gate BeadArray platform

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Global DNA Methylation: Uncommon Event in Oral Lichenoid Disease [Illumina GoldenGate Methylation Cancer Panel I array]

Project description:Purpose: Accumulating evidence indicates aberrant DNA methylation is closely related to oral carcinogenesis, and it has been shown that methylation changes might be used as prognostic biomarker in oral squamous cell carcinoma. Oral lichenoid disease (OLD) is the most common oral potentially malignant disorder in our region. In this study, we have performed a wide DNA methylation study of a series of oral lichenoid disease in order to assess the relevance of DNA methylation changes in this premalignant disorder. Experimental Design: Discovery phase utilized the Illumina Golden Gate Cancer Panel I in 51 OLD and 6 control samples. The differently methylated loci and the global DNA methylation surrogate LINE-1, were further validated in an independent sample set consisting in 160 OLD and 65 controls. Results: DNA methylation profiles of the OLD showed only minor significant differences when compared to controls. Conclusions: In summary, our data indicates that the frequency of aberrant DNA alteration is very low in OLD, which support the low rate of malignization of this oral potentially malignant disorder. Bisulphite-converted DNA from the 51 oral lichenoid disease samples and 6 control samples were hybridised to the Illumina GoldenGate Methylation Cancer Panel I.

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