Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

52 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The functional study of zebrafish Bad gene

ABSTRACT: Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-bad. This assay is used for determination of expression profiling at 24 hpf and 48 hpf under Bad deficiency. Two-condition experiment, wild type vs. MO-Bad treated cells. Biological replicates: each group contains 200 embryos.

ORGANISM(S): Danio rerio

SUBMITTER: gen lin

PROVIDER: E-GEOD-47971 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Zebrafish 22 hpf embryos: WT vs. MO-grnA injected

Project description:Transcriptional profiling of zebrafish embryos comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for the determination of expression profiling at 22 hpf under GRN-A deficiency. Two-condition experiment: wild type vs. MO-grnA treated cells. 3 biological replicates: each group contains 200 embryos.

2013-03-28 | E-GEOD-28318 | biostudies-arrayexpress

WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos

Project description:Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for determination of expression profiling of trunk muscle at 16, 24, 48, 72 hpf under GRN-A deficiency. Two-condition experiment, wild type vs. MO-grnA treated cells. Biological replicates: each group contains 200 embryos.

2013-06-01 | E-GEOD-38441 | biostudies-arrayexpress

The functional study of zebrafish Bad gene

Project description:Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-bad. This assay is used for determination of expression profiling at 24 hpf and 48 hpf under Bad deficiency.

2014-06-01 | GSE47971 | GEO

The functional study of zebrafish rig1 gene in NNV infection

Project description:Transcriptional profiling of ZF-4 cells comparing controle morpholino-infection cells with rig1 morpholino-infection cells. This assay is used for determination of gene expression profiling at 6 hpi under NNV infection in rig1 knockdown cells. Two-condition experiment, controle morpholino-NNV infection cells vs. rig1 morpholino-NNV infection cells (5 MOI).

2015-05-12 | E-GEOD-57599 | biostudies-arrayexpress

Gene expression profile in NNV infected ZF-4 cells

Project description:Transcriptional profiling of ZF-4 cells comparing mock infection cells with cells infected with NNV. This assay is used for determination of gene expression profiling at 6 hpi under NNV infection. Two-condition experiment, Mock infection vs. NNV infection (5 MOI).

2015-05-12 | E-GEOD-57600 | biostudies-arrayexpress

Zebrafish 22 hpf embryos: WT vs. MO-grnA injected

2013-03-28 | GSE28318 | GEO

WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos

2013-06-01 | GSE38441 | GEO

Assessment of hematopoietic failure due to RPL11 deficiency in a zebrafish model of DiamondM-bM-^@M-^SBlackfan anemia by deep sequencing

Project description:To comprehensively reflect the impact of RPL11 deficiency on the transcriptome of zebrafish embryos, we collected 40M-bM-^@M-^S50 RPL11-deficient and MO control zebrafish embryos at 48 hpf from separate experiments and constructed two mRNA-seq sequencing libraries in parallel. High-throughput sequencing was performed on the Hi-Seq2000 sequencing platform in parallel. The number of sequenced gene transcript reads was 35M-bM-^@M-^S40 million. We found that hemoglobin biosynthetic and hematological defects in RPL11-deficient zebrafish were related to dysregulation of iron metabolism-related genes, including tfa, tfr1b, alas2 and slc25a37, which are involved in heme and hemoglobin biosynthesis. In addition, we found reduced expression of the hematopoietic stem cells (HSC) marker c-myb and HSC transcription factor tal1 and hoxb4a in RPL11-deficient zebrafish embryos, indicating that the hematopoietic defects may be related to impaired HSC differentiation and proliferation. However, RPL11 deficiency did not affect the development of other blood cell lineages such as granulocytes and myelocytes. Compare 2 different transcriptomes of RPL11-deficient and MO control zebrafish embryos

2014-01-21 | E-GEOD-51326 | biostudies-arrayexpress

Transcription profiling of trf3 morpholino treated zebrafish embryos to identify Trf3 target genes

Project description:To identify Trf3 target genes, we performed expression profiling in trf3 MO-treated embryos (at 6 hpf) using a zebrafish oligonucleotide microarray representing ~12,800 genes.

2007-10-29 | E-MEXP-1279 | biostudies-arrayexpress

Zebrafish transcriptomics in 24 and 36 hpf embryos after knockdown of glucocorticoid receptor translation

Project description:To further characterize the roles of cortisol signaling via the glucocorticoid receptor (GR) in developing zebrafish, we have used morpholino oligonucleotides to knockdown GR protein translation and measured gene expression in RNA extracted from 24 and 36 hours post fertilization (hpf) embryos. The GR morpholino was characterized previously in Nesan et al., 2012, Endocrinology 181, 35-44) Single-cell zebrafish embryos were microinjected with either active morpholino or mispair control. Embryos were frozen at 24 and 36 hpf and total RNA extracted for microarray analysis. Three independent replicates (different breeding events on separate days) were performed for each treatment per timepoint.

2013-08-28 | E-GEOD-50376 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data