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Gene Expression Profile for knocking down Tet1 in pre-iPSCs culturing in Vc medium.

ABSTRACT: Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed RNAseq for WT and knocking down Tet1 in two pre-iPSCs cell lines, pre2-2 and pre3. Using two pre-iPSCs cell lines: pre2-2 and pre3, cultured by mES medium(+lif), transfected siCtrl/siTet1 siRNA or non-treatment, then, after 16h, the medium were replaced by mES(+lif) medium plus Vc(50ug/ml), total RNA was extracted after another 32h later for RNAseq.

ORGANISM(S): Mus musculus

SUBMITTER: Xiaoshan Wang

PROVIDER: E-GEOD-48206 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Vitamin C modulates TET1 function during somatic cell reprogramming.

Chen Jiekai J Guo Lin L Zhang Lei L Wu Haoyu H Yang Jiaqi J Liu He H Wang Xiaoshan X Hu Xiao X Gu Tianpeng T Zhou Zhiwei Z Liu Jing J Liu Jiadong J Wu Hongling H Mao Shi-Qing SQ Mo Kunlun K Li Yingying Y Lai Keyu K Qi Jing J Yao Hongjie H Pan Guangjin G Xu Guo-Liang GL Pei Duanqing D

Nature genetics 20131027 12

Vitamin C, a micronutrient known for its anti-scurvy activity in humans, promotes the generation of induced pluripotent stem cells (iPSCs) through the activity of histone demethylating dioxygenases. TET hydroxylases are also dioxygenases implicated in active DNA demethylation. Here we report that TET1 either positively or negatively regulates somatic cell reprogramming depending on the absence or presence of vitamin C. TET1 deficiency enhances reprogramming, and its overexpression impairs reprog ...[more]

PMID: 24162740

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