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S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice


ABSTRACT: The aim of the study was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-induced gene expression and locomotor sensitization in mice. Using mouse nucleus accumbens (NAc) tissue, whole-genome gene expression profiling revealed that repeated SAM treatment affected a limited number of genes, but significantly modified cocaine-induced gene expression by blunting nonspecifically the cocaine response. At the gene level, we discovered that SAM modulated cocaine-induced DNA methylation by inhibiting both promoter-associated CpG-island hyper- and hypomethylation in the NAc but not in the reference tissue cerebellum. Total RNA was extracted from the mouse nucleus accumbens (NAc) tissue. Two tissues were combined to a sample, 4 samples per group used. RNA quality and quantity were assessed using the Nano-Drop -1000 spectrophotometer and the Agilent 2100 Bioanalyzer.

ORGANISM(S): Mus musculus

SUBMITTER: Kaili Anier 

PROVIDER: E-GEOD-48365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice.

Anier Kaili K   Zharkovsky Alexander A   Kalda Anti A  

The international journal of neuropsychopharmacology 20130520 9


Several studies suggest that individual variability is a critical component underlying drug addiction as not all members of a population who use addictive substance become addicted. There is evidence that the overall epigenetic status of a cell (epigenome) can be modulated by a variety of environmental factors, such as nutrients and chemicals. Based on these data, our aim was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-  ...[more]

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