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Gene expression profiles of mammary epithelial and breast cancer cells following fever range hyperthermia

ABSTRACT: Preclinical and clinical studies have shown for decades that tumor cells demonstrate significantly enhanced sensitivity to “fever range” hyperthermia (increasing the intratumoral temperature to 42-45oC) than normal cells, although it is unknown why cancer cells exhibit this distinctive susceptibility. To address this issue, mammary epithelial cells and three malignant breast cancer lines were subjected to hyperthermic shock and microarray analysis of the global transcription changes was subsequently performed. MCF10A (mammary epithelial cells) and MCF7, MDA231, and MDA468 breast cancer cells were grow at normal growth temperatures or subjected to 30 minutes of hyperthermic (45oC) shock followed by replacement with conditioned media at normal growth temperatures. RNA was collected 4 hours after shock and subjected to microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Brad Bryan

PROVIDER: E-GEOD-48398 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiles of mammary epithelial and breast cancer cells following fever range hyperthermia

Project description:Preclinical and clinical studies have shown for decades that tumor cells demonstrate significantly enhanced sensitivity to “fever range” hyperthermia (increasing the intratumoral temperature to 42-45oC) than normal cells, although it is unknown why cancer cells exhibit this distinctive susceptibility. To address this issue, mammary epithelial cells and three malignant breast cancer lines were subjected to hyperthermic shock and microarray analysis of the global transcription changes was subsequently performed.

2013-12-01 | GSE48398 | GEO

HSF1 mediated Gene regulation in T cells at normal (37C) and febrile (40C) temperatures

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2012-09-20 | E-GEOD-41005 | biostudies-arrayexpress

HSF1 mediated Gene regulation in T cells at normal (37C) and febrile (40C) temperatures

2012-09-20 | GSE41005 | GEO

mRNA expression in ttcsp1 deletion mutant and wild type Thermus thermophilus HB8 strains grown on a rich medium

Project description:We observed the expression profile of the total mRNA of ttcsp1 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Keywords: temperature comparison Wild-type and ttcsp1 mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in the ttcsp1 mutant between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-15 | E-GEOD-12436 | biostudies-arrayexpress

RNASeq of HEK293 suspension cells treated with a 72 h hyperthermic shift to 40°C versus untreated (37°C) controls

Project description:Experiment aimed at understanding the cellular mechanisms leading to increased extracellular vesicle concentration under hyperthermic conditions. Human embryonic kidney (HEK293) cells were cultured 48 h under normal conditions (37°C/140 rpm/5% CO2) before being subjected to a 72 h, 40°C temperature shift.

2023-08-10 | E-MTAB-13273 | biostudies-arrayexpress

Transcriptome response to heat stress in the chicken hepatocellular carcinoma cell line

Project description:Background: Heat stress triggers an evolutionarily conserved set of responses in cells. The transcriptome responds to hyperthermia by altering expression of genes to adapt the cell or organism to survive the heat challenge. RNA-seq technology allows rapid identification of environmentally responsive genes on a large scale. In this study, we have used RNA -seq to identify heat stress responsive genes in the chicken male white-leghorn hepat ocellular (LMH) cell line. Result: The transcripts of 812 genes were responsive to heat stress (p <0.01) with 235 genes up- regulated and 577 down-regulated following 2.5 hours of heat stress. Among the up- regulated were genes whose products function as chaperones, along with genes aff ecting collagen synthesis and deposition, transcription factors, chromatin remodelers and genes modulating the WNT and TGF-beta pathways. Predominant among the down-regulated genes were ones that affect DNA replication and repair along with chromosom al segregation. Many of the genes identified in this study have not been previously implicated in the heat stress response. Conclusion: These data extend our understanding of the transcriptome response to heat stress. Many of the identified biological processes and pathways likely function in adapting cells and organisms to hyperthermic stress. This study may provide important guides to future efforts attempting to improve species abilities to withstand heat stress through genome wide association studies and breeding. In addition, the genes down regulated by heat stress may provide important targets for improving hyperthemic treatment in cancer patients. Cells were grown at either control ( 37oC) or heat stress (43oC) temperatures for 2.5 hours.

2015-02-24 | E-GEOD-55321 | biostudies-arrayexpress

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The cold shock response of Bacillus subtilis analyzed by RNA-sequencing: YplP regulates pyruvate transport at low temperatures

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2018-03-12 | GSE111577 | GEO

Measuring the dependence of the yeast heat shock response on intracellular pH and translation during stress

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RNASeq of HEK293 suspension cell-derived sEVs treated with a 72 h hyperthermic shift to 40°C versus untreated (37°C) controls

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