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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome and Translatome Profiling of Caco-2 cells

ABSTRACT: This experiment performed RNA-seq of transcriptome and translatome (translating mRNA) of Caco-2 cells We extracted transcriptome and translatome from Caco-2 cells and deep sequenced them

ORGANISM(S): Homo sapiens

SUBMITTER: Gong Zhang

PROVIDER: E-GEOD-48603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Resolving chromosome-centric human proteome with translating mRNA analysis: a strategic demonstration.

Zhong Jiayong J Cui Yizhi Y Guo Jiahui J Chen Zhipeng Z Yang Lijuan L He Qing-Yu QY Zhang Gong G Wang Tong T

Journal of proteome research 20131115 1

Chromosome-centric human proteome project (C-HPP) aims at differentiating chromosome-based and tissue-specific protein compositions in terms of protein expression, quantification, and modification. We previously found that the analysis of translating mRNA (mRNA attached to ribosome-nascent chain complex, RNC-mRNA) can explain over 94% of mRNA-protein abundance. Therefore, we propose here to use full-length RNC-mRNA information to illustrate protein expression both qualitatively and quantitativel ...[more]

PMID: 24200226

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