Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of animal-blastomeres' RNA content to vegetal-most half of vegetal blastomeres' RNA content at the 8-cell stage of Xenopus laevis embryos to identify animal-enriched transcripts


ABSTRACT: In Xenopus laevis, a number of studies identified vegetal factors that specify the germ line, endoderm and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in animal blastomeres. We sought to maximize differences between animal and vegetal samples. To that end, we dissected 8-cell embryos into animal blastomeres and vegetal blastomeres, and further dissected the vegetal blastomeres into vegetal-most halves (VP) and equatorial regions (discarded).

ORGANISM(S): Xenopus laevis

SUBMITTER: Sally Moody 

PROVIDER: E-GEOD-48659 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Novel animal pole-enriched maternal mRNAs are preferentially expressed in neural ectoderm.

Grant Paaqua A PA   Yan Bo B   Johnson Michael A MA   Johnson Diana L E DL   Moody Sally A SA  

Developmental dynamics : an official publication of the American Association of Anatomists 20131221 3


<h4>Background</h4>Many animals utilize maternal mRNAs to pre-pattern the embryo before the onset of zygotic transcription. In Xenopus laevis, vegetal factors specify the germ line, endoderm, and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in 8-cell-stage animal blastomeres.<h4>Results</h4>We identified 39 mRNAs isolated from 8-cell animal blastomeres  ...[more]

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