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Transcription profiling of mouse T65H translocation tissues at different developmental stages from mice with or without uniparental duplications of chromosomes 7 and 11 to identify novel imprinted genes

ABSTRACT: Tissue-specific comparison of gene expression levels in T65H translocation mice, either with or without uniparental duplications of Chrs 7 & 11. Identification of highly differentially expressed transcripts. Experiment Overall Design: Pairwise array comparative analyses were performed using MAS5/GCOS where sample age and tissue were matched, while the sample genotype varied between the compared arrays: matUpDp Chr 7 prox T65H vs either patUpDp Chr 7 prox T65H or normal (T65H translocation mouse w/o uniparental duplications), and matUpDp Chr 7 dist T65H vs normal.

ORGANISM(S): Mus musculus

SUBMITTER: Reiner Schulz

PROVIDER: E-GEOD-4870 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromosome-wide identification of novel imprinted genes using microarrays and uniparental disomies.

Schulz Reiner R Menheniott Trevelyan R TR Woodfine Kathryn K Wood Andrew J AJ Choi Jonathan D JD Oakey Rebecca J RJ

Nucleic acids research 20060719 12

Genomic imprinting refers to a specialized form of epigenetic gene regulation whereby the expression of a given allele is dictated by parental origin. Defining the extent and distribution of imprinting across genomes will be crucial for understanding the roles played by imprinting in normal mammalian growth and development. Using mice carrying uniparental disomies or duplications, microarray screening and stringent bioinformatics, we have developed the first large-scale tissue-specific screen fo ...[more]

PMID: 16855283

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Expression data from T65H translocation mice

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