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Comparison of gene expression profile in RAG2+ B lineage cells from the small intestinal lamina propria and RAG2+ B lineage cells from the bone marrow

ABSTRACT: We used a RAG2-GFP reporter mouse to show that RAG+ B lineage cells can be found in the small intestinal lamina proria in normally-housed mice at weaning age. We used microarry expression analysis to compare the RAG2+ population in the gut to the RAG2+ B lineage population in the bone marrow. Microarray was used to compare RAG2+ lamina propria B cells to RAG2+ bone marrow B cells. For each experiment, RAG2-GFP+ cells from 8-12 post-natal day 17-25 RAG2-GFP reporter mice were sorted from small intestinal lamina propria lymphocyte preparations and bone marrow. RNA extracted from trizol was used for the analysis. 3 independent replicates were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Frederick Alt

PROVIDER: E-GEOD-48805 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microbial colonization influences early B-lineage development in the gut lamina propria.

Wesemann Duane R DR Portuguese Andrew J AJ Meyers Robin M RM Gallagher Michael P MP Cluff-Jones Kendra K Magee Jennifer M JM Panchakshari Rohit A RA Rodig Scott J SJ Kepler Thomas B TB Alt Frederick W FW

Nature 20130821 7465

The RAG1/RAG2 endonuclease (RAG) initiates the V(D)J recombination reaction that assembles immunoglobulin heavy (IgH) and light (IgL) chain variable region exons from germline gene segments to generate primary antibody repertoires. IgH V(D)J assembly occurs in progenitor (pro-) B cells followed by that of IgL in precursor (pre-) B cells. Expression of IgH μ and IgL (Igκ or Igλ) chains generates IgM, which is expressed on immature B cells as the B-cell antigen-binding receptor (BCR). Rag expressi ...[more]

PMID: 23965619

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